Please use this identifier to cite or link to this item: http://dspace.cus.ac.in/jspui/handle/1/6421
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNajar, Ishfaq Nabi-
dc.contributor.authorSherpa, Mingma Thundu-
dc.contributor.authorDas, Sayak-
dc.contributor.authorDas, Saurav-
dc.contributor.authorThakur, Nagendra-
dc.date.accessioned2019-10-22T10:15:26Z-
dc.date.available2019-10-22T10:15:26Z-
dc.date.issued2018-
dc.identifier.citationScience of The Total Environment, V.637–638, 2018,730-745 pp.en_US
dc.identifier.issn0048-9697-
dc.identifier.urihttps://doi.org/10.1016/j.scitotenv.2018.05.037-
dc.identifier.urihttp://dspace.cus.ac.in/jspui/handle/1/6421-
dc.description.abstractNortheastern regions of India are known for their floral and faunal biodiversity. Especially the state of Sikkim lies in the eastern Himalayan ecological hotspot region. The state harbors many sulfur rich hot springs which have therapeutic and spiritual values. However, these hot springs are yet to be explored for their microbial ecology. The development of neo generation techniques such as metagenomics has provided an opportunity for inclusive study of microbial community of different environment. The present study describes the microbial diversity in two hot springs of Sikkim that is Polok and Borong with the assist of culture dependent and culture independent approaches. The culture independent techniques used in this study were next generation sequencing (NGS) and Phospholipid Fatty Acid Analysis (PLFA). Having relatively distinct geochemistry both the hot springs are thermophilic environments with the temperature range of 50–77 °C and pH range of 5–8. Metagenomic data revealed the dominance of bacteria over archaea. The most abundant phyla were Proteobacteria and Bacteroidetes although other phyla were also present such as Acidobacteria, Nitrospirae, Firmicutes, Proteobacteria, Parcubacteria and Spirochaetes. The PLFA studies have shown the abundance of Gram Positive bacteria followed by Gram negative bacteria. The culture dependent technique was correlative with PLFA studies. Most abundant bacteria as isolated and identified were Gram-positive genus Geobacillus and Anoxybacillus. The genus Geobacillus has been reported for the first time in North-Eastern states of India. The Geobacillus species obtained from the concerned hot springs were Geobacillus toebii, Geobacillus lituanicus, Geobacillus Kaustophillus and the Anoxybacillus species includes Anoxybacillus gonensis and Anoxybacillus Caldiproteolyticus. The distribution of major genera and their statistical correlation analyses with the geochemistry of the springs predicted that the temperature, pH, alkalinity, Ca2+, Mg2+, Cl2+, and sulfur were main environmental variables influencing the microbial community composition and diversity. Also the piper diagram suggested that the water of both the hot springs are Ca-HCO3– type and can be predicted as shallow fresh ground waters. This study has provided an insight into the ecological interaction of the diverse microbial communities and associated physicochemical parameters, which will help in determining the future studies on different biogeochemical pathways in these hot springs.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectHot springsen_US
dc.subjectMetagenomicsen_US
dc.subjectPhospholipid Fatty Acid Analysis (PLFA)en_US
dc.subjectInductively Coupled Plasma Mass Spectroscopy (ICPMS)en_US
dc.subjectGeobacillusen_US
dc.titleMicrobial ecology of two hot springs of Sikkim: predominate population and geochemistryen_US
dc.typeArticleen_US
dc.identifier.Volume637-638-
Appears in Collections:Nagendra Thakur

Files in This Item:
File Description SizeFormat 
1-s2.0-S0048969718316693-main.pdf3.87 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.