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dc.contributor.authorSathyanarayana, N.-
dc.contributor.authorPittala, Ranjith Kumar-
dc.contributor.authorTripathi, Pankaj Kumar-
dc.contributor.authorChopra, Ratan-
dc.contributor.authorSingh, Heikham Russiachand-
dc.contributor.authorBelamkar, Vikas-
dc.contributor.authorBhardwaj, Pardeep Kumar-
dc.contributor.authorDoyle, Jeff J.-
dc.contributor.authorEgan, Ashley N.-
dc.date.accessioned2019-10-17T10:39:07Z-
dc.date.available2019-10-17T10:39:07Z-
dc.date.issued2017-
dc.identifier.citationBMC Genomics,V.18(409),2017en_US
dc.identifier.issn1471-2164-
dc.identifier.urihttps://doi.org/10.1186/s12864-017-3780-9-
dc.identifier.urihttp://dspace.cus.ac.in/jspui/handle/1/6402-
dc.description.abstractBackground: The medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson’s drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M. pruriens, including a de novo transcriptome assembly and annotation, as well as differential transcript expression analyses between root, leaf, and pod tissues. We also develop microsatellite markers and analyze genetic diversity and population structure within a set of Indian germplasm accessions. Results: One-hundred ninety-one million two hundred thirty-three thousand two hundred forty-two bp cleaned reads were assembled into 67,561 transcripts with mean length of 626 bp and N50 of 987 bp. Assembled sequences were annotated using BLASTX against public databases with over 80% of transcripts annotated. We identified 7,493 simple sequence repeat (SSR) motifs, including 787 polymorphic repeats between the parents of a mapping population. 134 SSRs from expressed sequenced tags (ESTs) were screened against 23 M. pruriens accessions from India, with 52 EST-SSRs retained after quality control. Population structure analysis using a Bayesian framework implemented in fastSTRUCTURE showed nearly similar groupings as with distance-based (neighbor-joining) and principal component analyses, with most of the accessions clustering per geographical origins. Pair-wise comparison of transcript expression in leaves, roots and pods identified 4,387 differentially expressed transcripts with the highest number occurring between roots and leaves. Differentially expressed transcripts were enriched with transcription factors and transcripts annotated as belonging to secondary metabolite pathways. Conclusions: The M. pruriens transcriptomic resources generated in this study provide foundational resources for gene discovery and development of molecular markers. Polymorphic SSRs identified can be used for genetic diversity, marker-trait analyses, and development of functional markers for crop improvement. The results of differential expression studies can be used to investigate genes involved in L-Dopa synthesis and other key metabolic pathways in M. pruriens.en_US
dc.language.isoenen_US
dc.publisherBMCen_US
dc.subjectVelvet beanen_US
dc.subjectMucuna pruriensen_US
dc.subjectTranscriptomicsen_US
dc.subjectDifferential gene expressionen_US
dc.subjectEST-SSRsen_US
dc.subjectPopulation structureen_US
dc.subjectLeguminosaeen_US
dc.subjectFabaceaeen_US
dc.titleTranscriptomic resources for the medicinal legume Mucuna pruriens: de novo transcriptome assembly, annotation, identification and validation of EST-SSR markersen_US
dc.typeArticleen_US
dc.identifier.Volume18-
Appears in Collections:N. Sathyanarayana

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