000			07085nam a2200145Ia 4500
999			_c177047 _d177047
020			_a0805305734
040			_cCUS
082			_a579.078 _bCAP/M
245		0	_aMicrobiology/ _ba laboratory manual _cCappuccino, James G.
250			_a4th ed.
260			_aMenlo Park,: _bBenjamin/Cummings Pub. Co., _c1996.
300			_a477 p.
505			_a Part I Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms 1 Introduction 1 Experiment 1: Culture Transfer techniques 7 Experiment 2: Techniques for Isolation of Pure Cultures 13 Part A: Isolation of Discrete Colonies frbm a Mixed Culture 13 Part B: Isolation of Pure Cultures from a Spread-Plate or Streak-Plate Preparation 14 Experiment 3: Cultural Characteristics of Microorganisms 21 Part II Microscopy 27 Introduction 27 Experiment 4: Microscopic Examination of Stained Cell Preparations 29 Experiment 5: Microscopic Examination of Living Bacterial Preparations 37 Experiment 6: The Microscopic Measurement of Microorganisms 41 Part III Bacterial Staining 45 Introduction 45 Experiment 7: Simple Staining 51 Experiment 8: Negative Staining 55 Experiment 9: Gram Stain 59 Experiment 10: Acid-Fast Stain (Ziehl-Neelsen Method) 65 Experiment 11: Spore Stain (Schaeffer-Fulton-Method) 71 Experiment 12: Capsule Stain 75 Part IV Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations 79 Introduction 79 Experiment 13: Nutritional Requirements: Media for the Routine Cultivation of Bacteria 83 . * Experiment 14: Use of Differential and Selective Media 89 Experiment 15: Physical Factors: Temperature 95 ' Experiment 16: Physical Factors: pH of the Extracellular Environment 99 Xiv CONTENTS Experiment 17: Physical Factors: Atmospheric Oxygen Requirements 103 Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms 109 Experiment 19: Serial Dilution-Agar Plate Procedure to'Quantitate Viable Cells 115 Experiment 20: The Bacterial Growth Curve 121 Part V Biochemical Activities of Microorganisms 129 Introduction 129 Experiment 21: Extracellular Enzymatic Activities of Microorganisms 131 Experiment 22: Carbohydrate Fermentation 137 Experiment 23: Triple Sugar-Iron Agar Test 1.43 Experiment 24: IMViC Test 147 Part A: Indole Production Te^ 147 Part B: Methyl Red Test 148 Part C: Voges-Proskauer Test 149 Part D: Citrate Utilization Test 150 Experiment 25: Hydrogen Sulfide Test 157 Experiment 26: Urease Test 161 Experiment 27: Litmus Milk Reactions 165 Experiment 28: Nitrate Reduction Test 171 Experiment 29: Catalase Test 175 Experiment 30: Oxidase Test 179 Experiment 31: Genus Identification of Unknown Bacterial Cultures 183 Part VI The Protozoa 187 Introduction 187 Experiment 32: Free-Living Protozoa 189 • Experiment 33: Parasitic Protozoa 193 Part VII The Fungi 199 Introduction 199 Experiment 34: Cultivation and Morphology of Molds 201 Experiment 35: Yeast Morphology 205 Experiment 36: Identification of Unknown Fungi 209 Part VIII The Viruses 215 Introduction 215 Experiment 37: Cultivation and Enumeration of Bacteriophages 219 Experiment 38: Isolation of Coliphages from Raw Sewage 225 Experiment 39: Staphylococcal Phage Typing 231 Part IX Physical and Chemical Agents for the Control of CONTENTS Microbial Growth 235 Introduction 235 Experiment 40: Physical Agents of Control: Moist Heat 237 Experiment 41: Physical Agents of Control: Environmental Osmotic Pressure 245 XV Experiment 42: Physical Agents of Control: Electromagnetic Radiations 249 Experiment 43: Chemical Agents of Control: Chemotherapeutic Agents 253 Part A: The Kirby-Bauer Antimicrobial Susceptibility Test Procedure 254 Part B: Synergistic Effect of Drug Combinations 257 Experiment 44: Determination of Penicillin Activity in the Presence and Absence of Penicillinase 263 Experiment 45: Chemical Agents of Control: Disinfectants and Antiseptics 267 ■ i Part A: Phenol Coefficient 271 Part B: Agar Plate-Sensitivity Method 272 Part X Microbiology of Food 277 Introduction 277 Experiment 46: Methylene Blue Reductase Test 279 Experiment 47: Microbiological Analysis of Food Products: Bacterial Count 283 Experiment 48: Wine Production 287 Experiment 49: Sauerkraut Production 291 Part XI Microbiology of Water 295 Introduction 295 Experiment 50: Standard Qualitative Analysis of Water 297 Part A: Presumptive Test: Determination of the Most Probable Number of Coliform Bacteria 297 Part B: Confirmed Test 299 Part C: Completed Test 300 Experiment 51: Quantitative Analysis of Water: Membrane Filter Method 303 Part XII Microbiology of Soil 309 Introduction 309 Experiment 52: Nitrogen Cycle 311 Part A: Ammonification 311 Part B: Nitrification 312 Part C: Denitrification 313 Part D: Nitrogen Fixation 314 Experiment 53: Microbial Populations in Soil: Enumeration 323 Experiment 54: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates 329 Part A: Isolation of Antibiotic-Producing Microorganisms 329 Part B: Determination of Antimicrobial Spectrum of Isolates 331 . Experiment 55: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique 335 Part XIII Bacterial Genetics 341 introduction 341 Experiment 56: Enzyme Induction 343 Experiment 57: Bacterial Conjugation 347 Vi CONTENTS Experiment 58: Isolation of a Streptomycin-Resistant Mutant 353 Experiment 59: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity 357 Part XIV Medical Microbiology 363 Introduction 363 Experiment 60: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Experiment 61: Normal Microbial Flora of the Throat and Skin 369 Experiment 62: Identification of Human Staphylococcal Pathogens 373 Experiment 63: Identification of Human Streptococcal Pathogens 381 Experiment 64: Identification of Streptococcus pneumoniae 387 Experiment 65: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems 391 Enterotube Multitest System and Encise II 392 API (Analytical Profile Index) System 393 Experiment 66: Isolation and Presumptive Identification of Campylobacter 401 Experiment 67: Microbiological Analysis of Urine Specimens 405 Experiment 68: Microbiological Analysis of Blood Specimens 411 Experiment 69: Species Identification of Unknown Bacterial Cultures 417 Part XV Immunology 425 CONTENTS Introduction 425 Experiment 70: Precipitin Reaction: The Ring Test 427 Experiment 71: Precipitin Reaction: Immunodiffusion 431 Experiment 72: Agglutination Reaction: The Febrile Antibody Test 437 Experiment 73: Immunofluorescence 441 Experiment 74: Latex Agglutination Test 445 Experiment 75: Enzyme-Linked Immunosorbent Assay 449 Experiment 76. Agglutination Reaction: Mono-Test for Infectious Mononucleosis 453 Experiment 77: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures 457 Part A: Rapid Plasma Reagin Test for Syphilis 457 Part B: Genital Herpes: Isolation and Identification of Herpes Simplex Virus 458 Part C: Detection of Sexually Transmitted Chlamydial Diseases 459
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