000			00388nam a2200145Ia 4500
999			_c176429 _d176429
020			_a9789382291763
040			_cCUS
082			_a630 _bSMI/P
245		0	_aPlant tissue culture: techniques and experiments/ _cSmith, Roberta H.
250			_a3rd ed.
260			_aAmsterdam: _bElsevier, _c2013.
300			_axvi,188p.
505			_a Scheduling and Interrelationships of Exercises History of Plant Cell Culture Trevor A. Thorpe Introduction The Early Years The Era of Techniques Development The Recent Past Cell Behavior Plant Modification and Improvement Pathogen-Free Plants and Germplasm Storage Clonal Propagation Product Formation The Present Era Setup of a Tissue Culture Laboratory Work Areas Media Preparation/Culture Evaluation/Record-Keeping Area Aseptic Transfer Area Environmentally Controlled Culture Area Media Components and Preparation Inorganic Salts Plant Growth Regulators Vitamins Carbohydrates Hexitols Gelling Agent Amino Acids Antibiotics Natural Complexes Media pH Medium Preparation VI Contents 4. Explant Preparation 45 Explant Age 46 Season 46 Explant Size 46 Plant Quality 46 Goal 46 Genotype 48 Aseptic Technique 48 Aseptic Germination of Seeds 49 Carrot, Sunflower, Broccoli, Cotton, or Any Seed of Choice 49 Douglas Fir 50 5. Contamination 53 Explant 53 Disinfecting Agents 54 Explant Source 56 Internal Microbial Contamination 56 Explant Pretreatment to Control Microbial Contaminants 57 Personnel 57 Laminar Air Flow Hood 58 Insects 58 Control of Insect Contamination 58 Media 59 Instruments 60 Room Air Handling System 61 6. Callus Induction 63 Callus Initiation 63 Explant Preparation 65 Culture 66 Observations 66 Data and Questions 66 Explant Orientation 67 Explant 67 Observations 68 Establishment of Competent Cereal Cell Cultures 69 Explants 70 Data and Questions 70 Salt Selection In Vitro 70 Explants 71 Data and Questions 72 Growth Curves 72 Cotton 73 Tobacco 74 Cellular Variation from Callus Cultures 76 Contents vil 8. 9. Explant 76 Data and Questions 76 Regeneration and Morphogenesis 81 Controlled Morphogenesis: Potato Tuberization 82 Explant 83 Data and Questions 84 Somatic Embryogenesis 84 Explant Preparation 86 Procedure 86 Data and'Questions 86 Regeneration of Rice 87 Regeneration 87 Data and Questions 87 Dormancy Requirements of Explants 87 Explant Preparation 88 Questions 89 Primary Morphogenesis: Douglas Fir 89 Explant Preparation 89 Data and Questions 90 Woody Shrubs and Trees 93 Brent H. McCown Rhododendrons and Azaleas ^5 Explants 97 Advanced Studies 97 Roses 97 Explant 98 Advanced Studies 98 Birch Trees 98 Explant 98 Advanced Studies 99 White Cedar (Arborvitae) 99 Explant 99 Haploid Plants from Anther Culture 103 Datura Anther Culture 105 Explant Preparation 105 Questions 106 African Violet 106 Explant Preparation 107 Observations 107 Root Tip Chromosome Squash Technique 107 Questions 108 Tobacco 108 VIII 10. Explant Preparation 108 Observations 109 Questions 109 Anther Squash Technique 109 Embryo Rescue 113 Sweet Corn Embryo Culture 114 Preparation and Culture of Corn Embryos 115 Questions Crabapple and Pear 116 Explant Preparation Observations Questions Meristem Culture for Virus-Free Plants 119 Isolation of the Shoot Apical Meristem Explant Preparation Questions Establishing Virus- and Bacteria-Free Plants 122 Explant Preparation 122 Garlic Propagation from Bulb Scales 123 Explant Preparation 123 Data 123 In Vitro Propagation for Commercial Production of Ornamentals 127 Boston Fern Stage 1 Stage 1! Stage 111 Staghorn Fern 133 Stage 1 Stage II 134 Stage HI 135 Ficus 136 Stage ! 136 Stage II Stage III 137 Kalanchoe Explant Preparation 138 Data and Questions 138 African Violet 139 Explant Preparation 139 Data and Questions 140 Endangered Species Pitcher Plants 141 Cactus -|4-| Explant Preparation 142 13. Protoplast Isolation and Fusion 147 Jungeun Kim Park, Sunghun Park and James E. Craven Plant Protoplast Isolation and Fusion 148 Supplies 148 Protoplast Liberation 149 Protoplast Purification 150 Vital Staining 151 Protoplast Fusion 151 Yield Determination 15I Developing a Protoplast Isolation Protocol 152 14. /\grobacfer/t/m-Mediated Transformation of Plants 155 Jungeun Kim Park, Sunghun Park, Qingyu Wu and Stuart Sprague Petunia or Tobacco Leaf Disk ^ I57 Sequence I57 Preculture 157 Explant Preparation I57 Cocultivation with Agrobacterium 158 Shoot Transformation and Elimination Agrobacterium 158 Rooting and Pretesting For Transformed Shoots 159 Petunia Shoot Apex 160 Sequence 160 Seed Germination 160 Shoot Induction 161 Shoot Apex Transformation 161 Rooting 161 Tobacco Leaf Infiltration 162 Sequence * 162 Crow Agrobacterium 162 Culture Agrobacterium 163 Harvest Agrobacterium 163 Infiltration Tobacco Leaves 163
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