Basic methods in protein purification and analysis: a laboratory manual/ edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis
Material type: TextPublication details: New York: Cold Spring Harbor Laboratory Press, 2009Edition: 1st edDescription: vii, 436 p.: ill.; 28 cmISBN: 9780879698676Subject(s): Proteins-Purification | Proteins-AnalysisDDC classification: 572.6078Item type | Current library | Call number | Status | Notes | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|---|
General Books Science Library | Science Library, Sikkim University Science Library General Section | 572.6078 SIM/B (Browse shelf(Opens below)) | Available | Books For SU Science Library | P03057 |
1 Introduction to protein purification strategies
2 Preparation of cellular and subcellular extracts
Section 1: Purification using gel electrophoresis
3 One-dimensional polyacrylamide gel electrophoresis
4 Prparative two-dimensional gel electrophoresis with immobilized
Section 2: Purification using column chromatography
5 Ion-exchange chromatography
6 Size-exclusion chromatography
7 Reversed-phase high-performance liquid chromatography
8 Affinity and immunoaffinity chromatograpphy
9 Metal chelate affinity chromatography
10 Multidimensional chromatography of intact proteins
Section 3: Characterizing protein complexes
11 Identification of protein-protein interactions by conventional column chromatography
12 Identification of associated proteins by coimmunoprecipitation
13 Chromatin immunoprecipitation of protein complexe
14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins
15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
16 In silico tools: analysis for creating focused interaction networks
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