Principles of fluorescence spectroscopy/ Joseph R. Lakowicz.

Includes bibliographical references and index.

Introduction to Fluorescence -- Instrumentation for Fluorecence Spectroscopy -- Fluorophores -- Time-Domain Lifetime Measurements -- Frequency-Domain Lifetime Measurements -- Solvent and Environmental Effects -- Dynamics of Solvent and Spectral Relaxation -- Quenching of Fluorescence -- Mechanisms and Dynamics of Fluorescence Quenching -- Fluorescence Anisotropy -- Time-Dependent Anisotropy Decays -- Advanced Anisotropy Concepts -- Energy Transfer -- Time Resolved Energy Transfer and Conformational Distributions of Biopolymers -- Energy Transfer to Multiple Acceptors, in One-, Two- or Three-Dimensions -- Protein Fluorescence -- Time-Resolved Protein Fluorescence -- Multiphoton Excitation and Microscopy -- Fluorescence Sensing -- Novel Fluorophobes -- DNA Technology -- Fluorescence Lifetime Imaging Microscopy -- Single-Molecule Detection -- Fluorescence Correlation Spectroscopy -- Radiative Decay Engineering: Metal-Enhanced Fluorescence -- Radiative Decay Engineering: Surface Plasmon-Coupled Emission -- Appendix I. Corrected Emission Spectra -- Appendix II. Fluorescent Lifetime Standards -- Appendix III. Additional Reading -- Answers to Problems -- Index.