Protein purification/ priciples and practice
Scopes, Robert K.
- 3rd ed.
- New York: Springer-Verlag, 1994.
- 380 p.
Protein purification laboratory -- Making an extract -- Analysis: measurement of protein and enzyme activity -- Separation by precipitation -- Separation by adsorption I: general aspects -- Separation by adsorption II: ion exchangers and nonspecific adsorbents -- Separation by adsorption: affinity techniques -- Separation in solution -- Purification of special types of proteins -- Small-scale and large-scale procedures -- Analysis for purity -- Optimization of procedures: final steps -- Precipitation tables -- Solutions for measuring protein concentration -- Buffers for use in protein chemistry -- Chromatographic materials.