Comprehensive biotechnology Vol. 1/ principles and practices in industry agriculture medicine and the environment
Moo-Young,Murray
- Amsterdam: Elsevier, 2011.
- 690
General Preface
Nomenclature Guidelines
Permission Acknowledgments
1.01. Introduction
1.02. Amino Acid Metabolism
Glossary
1.02.1. Introduction
1.02.2. General Properties, Classification, and Structure of Amino Acids
1.02.3. Biosynthesis of Amino Acids
1.02.4. Catabolism of Amino Acids
1.02.5. Important Biomolecules Synthesized from Amino Acids
1.03. Enzyme Biocatalysis
Glossary
1.03.1. Introduction to Enzymes
1.03.2. Enzyme Kinetics
1.03.3. Enzyme Engineering
1.03.4. Enzyme Production
1.03.5. Immobilized Enzymes
1.03.6. Enzyme Applications
1.03.7. Conclusions
1.04. Immobilized Biocatalysts
1.04.1. Introduction: Definitions and Scope
1.04.2. Applications of Immobilized Enzymes
1.04.3. Methods of Enzyme Immobilization
1.04.4. Properties of Immobilized Enzymes
1.04.5. Evaluation of Enzyme Immobilization
1.04.6. Heterogeneous Biocatalysis
1.04.7. Future Prospects for Immobilized Biocatalysts
1.05. Lipids, Fatty Acids
Glossary
1.05.1. Introduction
1.05.2. Structure of Fatty Acids
1.05.3. Nomenclature
1.05.4. Form in the Cell
1.05.5. What Do Lipids Do?
1.05.6. Biosynthesis of Fatty Acids and Lipids
1.05.7. Biochemistry of Lipid Accumulation
1.06. DNA Cloning in Plasmid Vectors
Glossary
1.06.1. Introduction
1.06.2. Cloning Vectors: Replication Origins and Partition Regions
1.06.3. Cloning Vectors: Selection Markers
1.06.4. Preparing DNA Fragments for Ligation
1.06.5. Ligation Systems
1.06.6. Methods of Bacterial and Yeast Transformation
1.06.7. Exploitation of Bacteriophage Packaging for DNA Cloning in Plasmid Vectors
1.06.8. Screening of Plasmid Clones in Bacteria for the Desired Recombinant Plasmids
1.06.9. Vector-Implemented Systems for the Direct Selection of Recombinant Plasmids
1.06.10. Direct Selection of Recombinant Plasmids Involving Restriction Enzyme Digestion of the Ligation Mixture
1.06.11. Particular Features of Oligonucleotides’ Cloning
1.06.12. Particular Features of Cloning of PCR Amplicons
1.06.13. Introduction of Deletions into Plasmids
1.06.14. Instability of Recombinant Plasmids
1.06.15. DNA Cloning Using Site-Specific Recombination
1.06.16. DNA Cloning Using Homologous (General) Recombination
1.06.17. Employment of Transposons for In Vivo Cloning and Manipulation of Large Plasmids
1.06.18. Conclusion
1.07. Structure and Biosynthesis of Glycoprotein Carbohydrates
Glossary
Acknowledgments
1.07.1. Introduction
1.07.2. Monosaccharide Structure
1.07.3. Oligosaccharide Structure
1.07.4. Biosynthesis of Glycoproteins
1.07.5. Glycosylation of Therapeutic Glycoproteins
1.08. Nucleotide Metabolism
Glossary
1.08.1. Introduction
1.08.2. Synthesis of Phosphoribosyl Diphosphate (PRPP)
1.08.3. Purine Biosynthesis
1.08.4. Pyrimidine Biosynthesis
1.08.5. Nucleoside Triphosphate Formation
1.08.6. Deoxyribonucleotide Biosynthesis
1.08.7. Nucleotide Salvage
1.08.8. Purine and Pyrimidine Catabolism
1.08.9. Regulation of Gene Expression in Bacterial Nucleotide Synthesis
1.08.10. Exploitation of the Knowledge of Nucleotide Metabolism in Biotechnology
1.09. Organic Acids
Glossary
1.09.1. Introduction
1.09.2. Citric Acid
1.09.3. Gluconic Acid
1.09.4. Lactic Acid
1.09.5. Itaconic Acid
1.09.6. Other Acids
1.10. Peptides and Glycopeptides
Glossary
1.10.1. Introduction
1.10.2. Peptide Hormones
1.10.3. Neuropeptides
1.10.4. Antibacterial Peptides
1.10.5. Glycosylation Is a Common and Important Post-Translational Modification of Peptides
1.10.6. Common Glycosidic Linkages
1.10.7. Peptide Synthesis
1.10.8. Glycopeptide Synthesis
1.10.9. Peptides and Glycopeptides as Models of Proteins and Glycoproteins
1.10.10. Application of Synthetic Peptides and Glycopeptides for the Treatment of Disease
1.10.11. Summary
1.11. Protein Structural Analysis
Glossary
1.11.1. Introduction
1.11.2. Protein X-ray Crystallography
1.11.3. NMR Spectroscopy
1.11.4. Structure Analysis Using Intrinsic Protein Fluorescence
1.11.5. Conclusions
1.12. Secondary Metabolites
Glossary
1.12.1. Introduction
1.12.2. Antibiotics
1.12.3. Other Applications of Secondary Metabolites
1.13. Cell Line Isolation and Design
Glossary
1.13.1. Introduction
1.13.2. Clone Selection and Isolation
1.13.3. Automating Clone Screening
1.13.4. Designer Cell Lines for Bioproduction
1.13.5. Future Perspectives and Conclusions
1.14. Cell Preservation Technology
Glossary
1.14.1. Introduction
1.14.2. Hypothermic Storage
1.14.3. Hypothermic Continuum
1.14.4. Cryopreservation
1.14.5. Modes of Cell Death
1.14.6. Cell Death Continuum
1.14.7. Preservation-Induced Cell Death
1.14.8. Targeted Control of Molecular-Based Death
1.14.9. Concluding Thoughts
1.15. Cytoskeleton and Cell Motility
Glossary
1.15.1. Introduction
1.15.2. Myosins
1.15.3. Cell Migration
1.15.4. Involvement of Unconventional Myosins in Cell Migration and Trafficking
1.16. Design of Culture Media
Glossary
1.16.1. Introduction
1.16.2. Universal Requirements
1.16.3. Specific Requirements
1.16.4. Methods for Media Design
1.16.5. Manufacturing of the Designed Medium
1.16.6. Regulatory Considerations
1.16.7. Quality Control Testing
1.16.8. Security of Supply
1.16.9. Summary
1.17. Protein Folding in the Endoplasmic Reticulum
Glossary
Acknowledgments
1.17.1. Introduction: Protein Folding
1.17.2. The Endoplasmic Reticulum as a Folding, Assembly, and Trafficking Vehicle
1.17.3. Key Chaperones Assisting Folding in the ER
1.17.4. Calnexin and Calreticulin: Glycosylation and Glycoprotein Quality Control
1.17.5. PDI: Redox-Dependent Folding and Disulfide Bond Formation