TY  - BOOK
AU  - Parakhia, Manoj V. [et al.].
TI  - Molecular biology and biotechnology: Microbial methods
SN  - 9380235194
U1  - 572.8 
PY  - 2010///
CY  - New Delhi
PB  - NIPA
KW  - Molecular Biology
KW  - Biotechnology
N1  - 1. Laboratory safety rules
2. Milestones in DNA history and biotechnology
3. Contribution in development of DNA and biotechnology
4. Equipments required in a molecular marker laboratory
5. Basic calculations used in microbial biotechnology
6. Basic principals in preparation of reagents required in microbial biotechnology
7. Isolation of bacterial genomic DNA
8. Isolation of RNA from yeast
9. Nucleotide composition of RNA
10. DNA melting point determination
11. Quantitative determination of DNA by DPA method and spectroscopic method
12. Estimation of RNA by Orcinol method
13. Electrophoresis
14. SDS-PAGE electrophoresis
15. Polymerase chain reaction
16. Restriction enzyme digestion
17. Extraction of DNA fragments from Agarose Gel
18. Isolation the Mammalian DNA from whole blood
19. Isolation of Plasmid DNA from bacteria
20. Random Amplified Polymorphic DNA RAPD
21. Amplified Fragment Leanth Polymorphism AFLP
22. Southern blotting
23. Ligation of insert DNA to Vector DNA
24. Bacterial transformation
25. Detection of plasmid by transformation
26. Conjugation in E.Coli by plate method
27. Isolation of Lactose non fermentor mutants of E.Coli by Physical mutagenesis
28. Isolation of auxotrophic mutant by chemical 5 Bu mutagenesis
29. Mutagenesis direct selection
30. Mutagenesis indirect selection
31. Agrobacterium mediated gene transfer from bacteria to plant. 32. Western blotting
33. Northern blotting
34. PCR-sequencing
35. Overview of metagenomics
36. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0
ER  -