TY  - BOOK
AU  - edited by Simpson, Richard J.
AU  - Adams, Peter D.
AU  - Golemis, Erica A.
TI  - Basic methods in protein purification and analysis: a laboratory manual
SN  - 9780879698676
U1  - 572.6078 
PY  - 2009///
CY  - New York
PB  - Cold Spring Harbor Laboratory Press
KW  - Proteins-Purification
KW  - Proteins-Analysis
N1  - 1 Introduction to protein purification strategies
2 Preparation of cellular and subcellular extracts

Section 1: Purification using gel electrophoresis
3 One-dimensional polyacrylamide gel electrophoresis
4 Prparative two-dimensional gel electrophoresis with immobilized

Section 2: Purification using column chromatography
5 Ion-exchange chromatography
6 Size-exclusion chromatography
7 Reversed-phase high-performance liquid chromatography
8 Affinity and immunoaffinity chromatograpphy
9 Metal chelate affinity chromatography
10 Multidimensional chromatography of intact proteins

Section 3: Characterizing protein complexes
11 Identification of protein-protein interactions by conventional column chromatography 
12 Identification of associated proteins by coimmunoprecipitation
13 Chromatin immunoprecipitation of protein complexe
14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins
15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
16 In silico tools: analysis for creating focused interaction networks

ER  -