Basic methods in protein purification and analysis: a laboratory manual/
edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis
- 1st ed.
- New York: Cold Spring Harbor Laboratory Press, 2009.
- vii, 436 p.: ill.; 28 cm.
1 Introduction to protein purification strategies 2 Preparation of cellular and subcellular extracts
Section 1: Purification using gel electrophoresis 3 One-dimensional polyacrylamide gel electrophoresis 4 Prparative two-dimensional gel electrophoresis with immobilized
Section 2: Purification using column chromatography 5 Ion-exchange chromatography 6 Size-exclusion chromatography 7 Reversed-phase high-performance liquid chromatography 8 Affinity and immunoaffinity chromatograpphy 9 Metal chelate affinity chromatography 10 Multidimensional chromatography of intact proteins
Section 3: Characterizing protein complexes 11 Identification of protein-protein interactions by conventional column chromatography 12 Identification of associated proteins by coimmunoprecipitation 13 Chromatin immunoprecipitation of protein complexe 14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins 15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis 16 In silico tools: analysis for creating focused interaction networks