Yeast Genetics; Methods and Protocols/

1. Yeast Transformation by LiAc/SS Carrier DNA/PEG Method R. Daniel Gietz 2. Tetrad, Random Spore and Molecular Analysis of Meiotic Segregation and Recombination Michael Lichten 3. PCR Mutagenesis and Gap Repair in Yeast Mark Weir and Jill B. Keeney 4. PCR-Mediated Epitope Tagging of Genes in Yeast Radhika Mathur and Peter Kaiser 5. Manipulating the Yeast Genome: Deletion, Mutation and Tagging by PCR Jennifer M. Gardner and Sue L. Jaspersen 6. Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence Lucy F. Pemberton 7. Single Yeast Cell Imaging Heimo Wolinski and Sepp D. Kohlwein 8. Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells Anjali Bisaria, Pascal Hersen, and Megan N. McClean 9. Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations Brian Haarer and David C. Amberg 10. Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast Elena Kuzmin, Sara Sharifpoor, Anastasia Baryshnikova, Michael Costanzo, Chad L. Myers, Brenda J. Andrews, and Charles Boone 11. Chemical Genetic and Chemogenomic Analysis in Yeast Namal V. C. Coorey, Liam D.P. Sampson, Jacqueline M. Barber, and David S. Bellows 12. Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays John Rodgers, Jingyu Guo, and John L. Hartman, IV 13. Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast Badri Nath Singh and Michael Hampsey 14. Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast Maria D. Mayan and Luis Aragon 15. Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq Philippe Lefrancois, Jennifer E.G. Gallagher, and Michael Snyder 16. High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast David Lior, Sandra Clauder-Munster, and Lars M. Steinmetz 17. Analysis of Silencing in Saccharomyces cerevisiae Andrew Miller and Ann L. Kirchmaier 18. A User's Guide to the Ribosomal DANN in Saccharomysces cerevisiae Joseph M. Johnson, Jeffrey S. Smith, and David A. Schneider 19. Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication Sandra K. Villwock and Oscar M. Aparicio 20. Replicative Life Span Alaysis in Budding Yeast George L. Sutphin, Joe R. Delaney, and Matt Kaeberlein 21. Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast Vincent R. Richard, Simon D. Bourque, and Vladimir I. Titorenko