Molecular Biology and Genetic Engineering/ P.K. Gupta.

Part I Molecular Biology
1 Molecular Biology and Genetic Engineering : Definition, History and Scope
2 Chemistry of the Cell : 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids)
3 Chemistry of the Cell : 2. Macromolecules (Nucleic Acids, Proteins and Polysaccharides)
4 Chemistry of the Gene : Synthesis, Modification and Repair of DNA
5 Organization of Genetic Material : 1. Packaging of DNA as Nucleosomes in Eukaryotes
6 Organization of Genetic Material : 2. Repetitive and Unique DNA Sequences
7 Organization of Genetic Material : 3. Split Genes, Overlapping Genes, Pseudogenes and Cryptic genes
8 Multigene Families in Eukaryotes
9 Organization of Mitochondrial and Chloroplast Genomes
10 The Genetic Code
11 Protein Synthesis Apparatus : Ribosome, Transfer RNA and Aminoacyl -tRNS Synthetases
12 Expression of Gene : Protein Synthesis 1. Transcription in Prokaryotes and Eukaryotes
13 Expression of Gene : Protein Synthesis 2. RNA Processing (RNA Splicing, RNA Editing and Ribozymes)
14 Expression of Gene : Protein Synthesis 3. Synthesis and Transport of Proteins (Prokaryotes and Eukaryotes)
15 Regulation of Gene Expression 1. Operon Circuits in Bacteria and other Prokaryotes
16 Regulation of Gene Expression 2. Circuits for Lytic Cycle and Lysogeny in Bacteriophages
17 Regulation of Gene Expression 3. A Variety of Mechanisms in Eukaryotes (Including Cell Receptors and Cell Signalling)

PART II Genetic Engineering
18 Recombinant DNA and Gene Cloning 1. Cloning and Expression Vectors
19 Recombinant DNA and Gene Cloning 2. Chimeric DNA, Molecular Probes and Gene Libraries
20 Polymerase Chain Reaction (PCR) and Gene Amplification
21 Isolation, Sequencing and Synthesis of Genes
22 Proteins : Separation, Purification and Identification
23 Immunotechnology 1. B-Cells, Antibodies, Interferons and Vaccines
24 Immunotechnology 2. T -Cell Receptors and MHC Restriction
25 Immunotechnology 3. Hybridoma and Monoclonal Antibodies (mAbs)
26 Transfection Methods and Transgenic Animals
27 Animal and Human Genomics : Molecular Maps and Genome Sequences
28 Biotechnology In Medicine : 1. Vaccines, Diagnostics and Forensics
29 Biotechnology in Medicine : 2. Gene Therapy
30 Biotechnology in Medicine : 3. Pharmacogenetics / Pharmacogenomics and Personalized Medicine
31 Plant Cell and Tissue Culture : Production and Uses of Haploids
32 Gene Transfer Methods In Plants
33 Transgenic Plants : Genetically Modified (GM) Crops and Floricultural Plants
34 Plant Genomics : Molecular Maps and Genome Sequences
35 Genetically Engineered Microbes (GEMs) and Microbial Genomics