Neural Stem Cells: Methods and Protocols

Part I. Isolation and Culture of NSCs
1. Neural Differentiation of Embryonic Stem Cells / K. Sue O'Shea
2. Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens / Eric D. Laywell [and others]
3. Isolation of Stem and Precursor Cells from Fetal Tissue / Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao
4. Olfactory Ensheathing Cells : Isolation and Culture from the Rat Olfactory Bulb / Susan C. Barnett and A. Jane Roskams
5. Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium / Edmund Au and A. Jane Roskams
6. Production of Immortalized Human Neural Crest Stem Cells / Seung U. Kim [and others]
7. Adult Rodent Spinal Cord Derived Neural Stem Cells : Isolation and Characterization / Lamya S. Shihabuddin
8. Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood / Shijie Song and J. Sanchez-Ramos
9. Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers / Erin Lavik [and others]
Part II. Characterization of NSCs In Vitro
A. Cellular Techniques
10. Analysis of Cell Generation in the Telencephalic Neuroepithelium / Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide
11. Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures / Angelo L. Vescovi, Rossella Galli, and Angela Gritti
12. Assessing the Involvement of Telomerase in Stem Cell Biology / Mark P. Mattson, Peisu Zhang, and Weiming Fu
13. Detection of Telomerase Activity in Neural Cells / Karen R. Prowse
14. In Vitro Assays for Neural Stem Cell Differentiation / Marcel M. Daadi
15. Electron Microscopy and Lac-Z Labeling / Bela Kosaras and Evan Snyder
B. Electrophysiological Techniques
16. Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells / David S.K. Magnuson and Dante J. Morassutti
C. Molecular Techniques
17. Fluorescence In Situ Hybridization / Barbara A. Tate and Rachel L. Ostroff
18. RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells / Theresa E. Gratsch
19. Differential Display : Isolation of Novel Genes / Theresa E. Gratsch
20. Cell Labeling and Gene Misexpression by Electroporation / Terence J. Van Raay and Michael R. Stark
21. Gene Therapy Using Neural Stem Cells / Luciano Conti and Elena Cattaneo
22. Modeling Brain Pathologies Using Neural Stem Cells / Simonetta Siplone and Elena Cattaneo
Part III. Utilization/Characterization of NSCs In Vivo
A. Endogenous Pools of Stem/Progenitor Cells
23. Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model / Marcel M. Daadi
24. Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods / Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano
25. Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
Sanjay S.P. Magavi and Jeffrey D. Macklis283
26. Immunocytochemical Analysis of Neuronal Differentiation / Sanjay S.P. Magavi and Jeffrey D. Macklis
27. Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S.P. Magavi, and Jeffrey D. Macklis
B. Transplantation
28. Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore
29. Optimizing Stem Cell Grafting into the CNS / Scott R. Whittemore [and others]
30. Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos / Lorenzo Magrassi
31. Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells / Tanja Zigova and Mary B. Newman
32. Routes of Stem Cell Administration in the Adult Rodent / Alison E. Willing [and others]