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Plant tissue culture: techniques and experiments/ Smith, Roberta H.

Material type: Text

TextPublication details: Amsterdam: Elsevier, 2013Edition: 3rd edDescription: xvi,188pISBN: 9789382291763DDC classification: 630

Contents:

Scheduling and Interrelationships of Exercises History of Plant Cell Culture Trevor A. Thorpe Introduction The Early Years The Era of Techniques Development The Recent Past Cell Behavior Plant Modification and Improvement Pathogen-Free Plants and Germplasm Storage Clonal Propagation Product Formation The Present Era Setup of a Tissue Culture Laboratory Work Areas Media Preparation/Culture Evaluation/Record-Keeping Area Aseptic Transfer Area Environmentally Controlled Culture Area Media Components and Preparation Inorganic Salts Plant Growth Regulators Vitamins Carbohydrates Hexitols Gelling Agent Amino Acids Antibiotics Natural Complexes Media pH Medium Preparation VI Contents 4. Explant Preparation 45 Explant Age 46 Season 46 Explant Size 46 Plant Quality 46 Goal 46 Genotype 48 Aseptic Technique 48 Aseptic Germination of Seeds 49 Carrot, Sunflower, Broccoli, Cotton, or Any Seed of Choice 49 Douglas Fir 50 5. Contamination 53 Explant 53 Disinfecting Agents 54 Explant Source 56 Internal Microbial Contamination 56 Explant Pretreatment to Control Microbial Contaminants 57 Personnel 57 Laminar Air Flow Hood 58 Insects 58 Control of Insect Contamination 58 Media 59 Instruments 60 Room Air Handling System 61 6. Callus Induction 63 Callus Initiation 63 Explant Preparation 65 Culture 66 Observations 66 Data and Questions 66 Explant Orientation 67 Explant 67 Observations 68 Establishment of Competent Cereal Cell Cultures 69 Explants 70 Data and Questions 70 Salt Selection In Vitro 70 Explants 71 Data and Questions 72 Growth Curves 72 Cotton 73 Tobacco 74 Cellular Variation from Callus Cultures 76 Contents vil 8. 9. Explant 76 Data and Questions 76 Regeneration and Morphogenesis 81 Controlled Morphogenesis: Potato Tuberization 82 Explant 83 Data and Questions 84 Somatic Embryogenesis 84 Explant Preparation 86 Procedure 86 Data and'Questions 86 Regeneration of Rice 87 Regeneration 87 Data and Questions 87 Dormancy Requirements of Explants 87 Explant Preparation 88 Questions 89 Primary Morphogenesis: Douglas Fir 89 Explant Preparation 89 Data and Questions 90 Woody Shrubs and Trees 93 Brent H. McCown Rhododendrons and Azaleas ^5 Explants 97 Advanced Studies 97 Roses 97 Explant 98 Advanced Studies 98 Birch Trees 98 Explant 98 Advanced Studies 99 White Cedar (Arborvitae) 99 Explant 99 Haploid Plants from Anther Culture 103 Datura Anther Culture 105 Explant Preparation 105 Questions 106 African Violet 106 Explant Preparation 107 Observations 107 Root Tip Chromosome Squash Technique 107 Questions 108 Tobacco 108 VIII 10. Explant Preparation 108 Observations 109 Questions 109 Anther Squash Technique 109 Embryo Rescue 113 Sweet Corn Embryo Culture 114 Preparation and Culture of Corn Embryos 115 Questions Crabapple and Pear 116 Explant Preparation Observations Questions Meristem Culture for Virus-Free Plants 119 Isolation of the Shoot Apical Meristem Explant Preparation Questions Establishing Virus- and Bacteria-Free Plants 122 Explant Preparation 122 Garlic Propagation from Bulb Scales 123 Explant Preparation 123 Data 123 In Vitro Propagation for Commercial Production of Ornamentals 127 Boston Fern Stage 1 Stage 1! Stage 111 Staghorn Fern 133 Stage 1 Stage II 134 Stage HI 135 Ficus 136 Stage ! 136 Stage II Stage III 137 Kalanchoe Explant Preparation 138 Data and Questions 138 African Violet 139 Explant Preparation 139 Data and Questions 140 Endangered Species Pitcher Plants 141 Cactus -|4-| Explant Preparation 142 13. Protoplast Isolation and Fusion 147 Jungeun Kim Park, Sunghun Park and James E. Craven Plant Protoplast Isolation and Fusion 148 Supplies 148 Protoplast Liberation 149 Protoplast Purification 150 Vital Staining 151 Protoplast Fusion 151 Yield Determination 15I Developing a Protoplast Isolation Protocol 152 14. /\grobacfer/t/m-Mediated Transformation of Plants 155 Jungeun Kim Park, Sunghun Park, Qingyu Wu and Stuart Sprague Petunia or Tobacco Leaf Disk ^ I57 Sequence I57 Preculture 157 Explant Preparation I57 Cocultivation with Agrobacterium 158 Shoot Transformation and Elimination Agrobacterium 158 Rooting and Pretesting For Transformed Shoots 159 Petunia Shoot Apex 160 Sequence 160 Seed Germination 160 Shoot Induction 161 Shoot Apex Transformation 161 Rooting 161 Tobacco Leaf Infiltration 162 Sequence * 162 Crow Agrobacterium 162 Culture Agrobacterium 163 Harvest Agrobacterium 163 Infiltration Tobacco Leaves 163

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Item type	Current library	Call number	Status	Date due	Barcode	Item holds
General Books	Central Library, Sikkim University General Book Section	630 SMI/P (Browse shelf(Opens below))	Available		P31429

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Scheduling and Interrelationships of Exercises
History of Plant Cell Culture
Trevor A. Thorpe
Introduction
The Early Years
The Era of Techniques Development
The Recent Past
Cell Behavior
Plant Modification and Improvement
Pathogen-Free Plants and Germplasm Storage
Clonal Propagation
Product Formation
The Present Era
Setup of a Tissue Culture Laboratory
Work Areas
Media Preparation/Culture Evaluation/Record-Keeping Area
Aseptic Transfer Area
Environmentally Controlled Culture Area
Media Components and Preparation
Inorganic Salts
Plant Growth Regulators
Vitamins
Carbohydrates
Hexitols
Gelling Agent
Amino Acids
Antibiotics
Natural Complexes
Media pH
Medium Preparation
VI Contents
4. Explant Preparation 45
Explant Age 46
Season 46
Explant Size 46
Plant Quality 46
Goal 46
Genotype 48
Aseptic Technique 48
Aseptic Germination of Seeds 49
Carrot, Sunflower, Broccoli, Cotton, or Any Seed of Choice 49
Douglas Fir 50
5. Contamination 53
Explant 53
Disinfecting Agents 54
Explant Source 56
Internal Microbial Contamination 56
Explant Pretreatment to Control Microbial Contaminants 57
Personnel 57
Laminar Air Flow Hood 58
Insects 58
Control of Insect Contamination 58
Media 59
Instruments 60
Room Air Handling System 61
6. Callus Induction 63
Callus Initiation 63
Explant Preparation 65
Culture 66
Observations 66
Data and Questions 66
Explant Orientation 67
Explant 67
Observations 68
Establishment of Competent Cereal Cell Cultures 69
Explants 70
Data and Questions 70
Salt Selection In Vitro 70
Explants 71
Data and Questions 72
Growth Curves 72
Cotton 73
Tobacco 74
Cellular Variation from Callus Cultures 76
Contents vil
8.
9.
Explant
76
Data and Questions
76
Regeneration and Morphogenesis
81
Controlled Morphogenesis: Potato Tuberization 82
Explant 83
Data and Questions 84
Somatic Embryogenesis
84
Explant Preparation
86
Procedure 86
Data and'Questions
86
Regeneration of Rice
87
Regeneration
87
Data and Questions
87
Dormancy Requirements of Explants
87
Explant Preparation
88
Questions
89
Primary Morphogenesis: Douglas Fir
89
Explant Preparation
89
Data and Questions
90
Woody Shrubs and Trees 93
Brent H. McCown
Rhododendrons and Azaleas
^5
Explants
97
Advanced Studies
97
Roses
97
Explant
98
Advanced Studies
98
Birch Trees
98
Explant
98
Advanced Studies
99
White Cedar (Arborvitae)
99
Explant
99
Haploid Plants from Anther Culture
103
Datura Anther Culture
105
Explant Preparation
105
Questions
106
African Violet
106
Explant Preparation
107
Observations
107
Root Tip Chromosome Squash Technique
107
Questions
108
Tobacco
108
VIII
10.
Explant Preparation
108
Observations
109
Questions
109
Anther Squash Technique
109
Embryo Rescue
113
Sweet Corn Embryo Culture
114
Preparation and Culture of Corn Embryos
115
Questions
Crabapple and Pear
116
Explant Preparation
Observations
Questions
Meristem Culture for Virus-Free Plants
119
Isolation of the Shoot Apical Meristem
Explant Preparation
Questions
Establishing Virus- and Bacteria-Free Plants
122
Explant Preparation
122
Garlic Propagation from Bulb Scales
123
Explant Preparation
123
Data
123
In Vitro Propagation for Commercial Production of
Ornamentals
127
Boston Fern
Stage 1
Stage 1!
Stage 111
Staghorn Fern
133
Stage 1
Stage II
134
Stage HI
135
Ficus
136
Stage !
136
Stage II
Stage III
137
Kalanchoe
Explant Preparation
138
Data and Questions
138
African Violet
139
Explant Preparation
139
Data and Questions
140
Endangered Species
Pitcher Plants 141
Cactus -|4-|
Explant Preparation 142
13. Protoplast Isolation and Fusion 147
Jungeun Kim Park, Sunghun Park and James E. Craven
Plant Protoplast Isolation and Fusion 148
Supplies 148
Protoplast Liberation 149
Protoplast Purification 150
Vital Staining 151
Protoplast Fusion 151
Yield Determination 15I
Developing a Protoplast Isolation Protocol 152
14. /\grobacfer/t/m-Mediated Transformation of Plants 155
Jungeun Kim Park, Sunghun Park, Qingyu Wu and Stuart Sprague
Petunia or Tobacco Leaf Disk ^ I57
Sequence I57
Preculture 157
Explant Preparation I57
Cocultivation with Agrobacterium 158
Shoot Transformation and Elimination Agrobacterium 158
Rooting and Pretesting For Transformed Shoots 159
Petunia Shoot Apex 160
Sequence 160
Seed Germination 160
Shoot Induction 161
Shoot Apex Transformation 161
Rooting 161
Tobacco Leaf Infiltration 162
Sequence * 162
Crow Agrobacterium 162
Culture Agrobacterium 163
Harvest Agrobacterium 163
Infiltration Tobacco Leaves 163

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