Techniques in cancer research: a laboratory manual/ M.G. Deo, R. Seshadri, R. Mulherkar and A.N. Bhisey

Including index.

Content
Cell Biology -- Cell and Tissue Culture -- Primary cultures -- Cell transformation assays -- Chromosome preparation and banding techniques -- Autoradiography of cells and tissue sections -- Somatic cell hybridization --Cell cycle analysis -- Cell adhesion assay -- Flow cytometry and its use in biological systems -- Electron microscopy -- Biochemistry -- Cell and macromolecule separation -- Sub-cellular fractionation of liver tissue -- Radiolabelling of proteins and cells -- Protein purification -- Protein characterization -- Peptide mapping -- Amino acid analysis -- N- terminal amino acid sequencing of proteins -- Zymographic detection of proteases -- Plasma membrane studies with leetins -- Carbohydrate analysis -- Hormones and growth factors -- Molecular biology -- DNA extraction --Digestion of DNA with restriction endonucleases -- Southern hybridization analysis -- RNA extraction -- Northern blotting -- Polymerase Chain Reaction (PCR) -- Single strand conformation polymorphism (SSCP) analysis -- In-situ hybridization of mRNA -- Maintenance and storage of bacterial strains -- Transfromation of bacteria by plassmid DNA -- Small scale isolation of plasmid DNA -- Large scale perpartatiion of Plasmid DNA from bacterial cells -- Trandfecton of Mammalian cells with Plasmid DNA -- Cloning in M13 for nucleotide sequencing -- Nucleotide sequencing -- Oncogenes and suppressor genes -- Genetic Engineering -- Gene therapy -- Rapid retrovirus vector production in 293 cells -- Replication-Competent Retrovirus (RCR) detection -- VL30 detection by PCR -- Advenovirus vectors: CsCI purification -- Ademovirus vextors: Titration using plaque assay on 293v cells -- assay of B-galactosidase in cultured cells -- Bystander effect and anti-tumor activity -- Production of ransgenic mice by micro manipulation -- Virology -- Demonstration of viruses using nucleic acid probes -- Cultivation of human retroviruses -- Immunology -- Generation of polyclonal antibodies -- Purification of immunoglobulins -- Characterization of antibodies -- Immunoprecipitation -- Radioimmunoassay -- Enzyme linked immunsosorbent assay -- Cell ELISA -- Immunoflurorescence assay -- Immunodeficient staining techniques -- Hybridoma techniques -- Separation & isolation of blood cells -- T&B Lymphocyte surface markers -- Iymphocyte transformation -- Lymphokine activated killer (LAK) cells -- Functional assessment of effect or cells -- Bioassays for cytokines -- EBV transformed B-cell lines -- Establisment of T-cell clones -- Carcinogenesis -- Assessment of mutagenity and genotoxicity -- Assessment of carcinogenicity -- Analysis of tabacco and its products -- Assessment of human exposure of tobacco -- Measurement of exposure to alkylatic agents (Potential carcinogens) -- Measurement of detixifying enzymes -- Chemotherapy -- Screening of anti-cancer drugs -- Animal Sciences -- Laboratory animals-breeding, care and maintenance
Appendix
Index