Mass spectrometry and genomic analysis/ J. Nicholas Housby.
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Central Library, Sikkim University General Book Section | 572.86 HOU/M (Browse shelf(Opens below)) | Available | P08771 |
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572.86 CAM/D Discovering genomics, proteomics and bioinformatics/ | 572.86 EST Epigenetics in biology and medicine/ | 572.86 HAR/E Essential Genetics/ | 572.86 HOU/M Mass spectrometry and genomic analysis/ | 572.86 JUN/G Genome annotation/ | 572.86 KIE/D DNA Sequencing III/ | 572.86 NAK/D Drug-DNA interactions/ |
CHAPTER 1
TJ. Griffin, LM. Smith
Single-Nucleotide Polymorphism Analysis by MALDI-TOF Mass Spectrometry
1. Introduction
2. Analysis of Peptide Nucleic Acid Hybridisation Probes
3. Direct Analysis of Invasive Cleavage Products
4. Conclusions
5. Experimental Methods
6. Affiliations
7. References
CHAPTER 2
LA. Haff, AC. Belden. LR. Hall, PL. Ross, IP. Smirnov
SNP Genotyping by MALDI-TOF Mass Spectrometry
1. Introduction
2. SNP Analysis by Single Base Extension of Primers
3. Maerials and Methods
4. Design Considerations for the SNP Genotyping Assay
5. The Single Base Extension Reaction
6. Modification of the SNP Typing Assay to Support Allele
Frequency Determination
7. Conclusions
8. References
CHAPTER 3
Hubert Koster
MASSARRAY: Highly Accurate and Versatile High Throughput Analysis of Genetic Variations
1. Introduction
2. MassARRAY Technology
3. Methodologly of MassARRAY Technology
4. Diagnostic Applicatioins of MassARRAY Technology for
Analysis of DNA
5. Application of MassARRAY for Confirmation and Validation of
Single Nucleotide Polymorphisms
6. Conclusions
7. Materials and Methods
8. Acknowledgements
9. References
CHAPTER 4
S.Sauer, D. Lechner, IG. Gut
The GOOD Assay
1. Introduction
2. SNP Genotyping by MALDI
3. How to Imporve the Analysis of DNA by MALDI
4. Priciples of the GOOD Assay
5. Variations of the GOOD Assay
6. Materials and Method of the GOOD Assay
7. Applications of the GOOD Assay
8. The Issue of DNA Quality
9. Physical Haplotyping by the GOOD Assay
10. Quantitation
11. Automation of the GOOD Assay
12. Outlook
13. References
CHAPTER 5
PH. Tsatsos, V. Vasiliskov, A. Mirzabekov
Microchip Analysis of DNA Sequence by Contiguous Stacking of Oligonucleotides and Mass Spectrometry
1. Introduction
2. Magichip Properties
3. Hybridisation
4. Generic Microchip
5. Principle of Contiguous Stacking Hybridisation
6. Monitoring
7. Conclusions
8. Acknowledgements
9. References
CHAPTER 6
PE. Jackson, MD. Friesen, JD. Groopman
Short Oligonucleotide Mass Analysis (SOMA): an ESI-MS Application for Genotyping and Mutation Analysis
1. Introduction
2. Short Oligonucleotide Mass Analysis
3. Electrospray Ionisation Mass Spectrometry
4. Purification Procedures
5. Genotyping Using SOMA
6. Mutation Detection Using SOMA
7. Advantages and Disadvantages of SOMA
8. Future Perspectives
9. Acknowledgements
10. References
CHAPTER 7
WV. Bienvenut, M. Muller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, GJ. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser
Proteomics and Mass Spectrometry: Some Aspects and Recent Developments
1. Introduction to Proteomics
2. Protein Biochemical and Chemical Processing Followed by
Mass Spectrometric Analysis
3. Protein Identification Using Bioinformatics Tools
4. Bioinformatics Tools for the Molecular Scanner
5. Conclusions
6. Acknowledgements
7. References
Index
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