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Lab ref: a handbook of recipes, reagents, and other reference tools for use at the bench/ edited by Albert S. Mellick and Linda Rodgers

Contributor(s): Mellick, Albert S., ed | Rodgers, LindaMaterial type: Text

TextPublication details: New York: Cold Spring Harbor Laboratory Press, 2007Description: 2v. (x, 272 p.; xi, 248 p.) : ill. ; 24 cmISBN: 9780879698157; 0879696303Subject(s): BiochemistryDDC classification: 572.078

Contents:

Section 1; Quantitative nucleic acid analysis: Isolation of total DNA -- Isolation of total RNA -- Selection of Poly(A) RNA from total -- Isolation and amplification of RNA from single cells and small amounts of tissue -- Hybridization analysis -- Preparation of nucleic acid (target) for array hybridization analysis -- Preparation of nucleic acid (probe) for array hybridization analysis -- Other quantitative methods -- Section 2; RNAi: RNAi in C. elegans -- RNAi in D. melanogaster -- RNAi in plants -- RNAi in mammalians -- RNAi in mouse oocytes and early embryos -- Section 3; Imaging: Preparation of embryos for live cell imaging -- Imaging fixed tissue -- Preparation of mice for imaging -- Intracellular imaging -- General information for imaging -- Section 4; Protein analysis/proteomics: Basic methods in protein analysis for proteomics -- Extraction of proteins -- Advanced protocols in identifying protein-protein interactions for proteomics -- Section 5; Arabidopsis: Microscopy -- Genetic manipulation -- Protein extraction -- Nuclear extraction -- Histochemistry of GUS fusion -- Section 6; Murine: Invitro culturing of embryonic stem cells -- Genotyping -- Immunohistochemistry of whole-mount embryos -- Other buffers and solutions -- Section 7; Storage and shipment of biological samples -- Section 8; Useful facts and tables -- Nomenclature guide -- Useful World Wide Web sites.

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Holdings
Item type	Current library	Collection	Call number	Vol info	Status	Date due	Barcode	Item holds
Reference Books	Central Library, Sikkim University Reference	Reference Collection	572.078 MEL/L (Browse shelf(Opens below))	v. 2	Not For Loan		P03627

Total holds: 0

Section 1; Quantitative nucleic acid analysis: Isolation of total DNA --
Isolation of total RNA --
Selection of Poly(A) RNA from total --
Isolation and amplification of RNA from single cells and small amounts of tissue --
Hybridization analysis --
Preparation of nucleic acid (target) for array hybridization analysis --
Preparation of nucleic acid (probe) for array hybridization analysis --
Other quantitative methods --
Section 2; RNAi: RNAi in C. elegans --
RNAi in D. melanogaster --
RNAi in plants --
RNAi in mammalians --
RNAi in mouse oocytes and early embryos --
Section 3; Imaging: Preparation of embryos for live cell imaging --
Imaging fixed tissue --
Preparation of mice for imaging --
Intracellular imaging --
General information for imaging --
Section 4; Protein analysis/proteomics: Basic methods in protein analysis for proteomics --
Extraction of proteins --
Advanced protocols in identifying protein-protein interactions for proteomics --
Section 5; Arabidopsis: Microscopy --
Genetic manipulation --
Protein extraction --
Nuclear extraction --
Histochemistry of GUS fusion --
Section 6; Murine: Invitro culturing of embryonic stem cells --
Genotyping --
Immunohistochemistry of whole-mount embryos --
Other buffers and solutions --
Section 7; Storage and shipment of biological samples --
Section 8; Useful facts and tables --
Nomenclature guide --
Useful World Wide Web sites.

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