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Basic methods in protein purification and analysis: a laboratory manual/ edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis

Contributor(s): edited by Simpson, Richard J | Adams, Peter D | Golemis, Erica AMaterial type: Text

TextPublication details: New York: Cold Spring Harbor Laboratory Press, 2009Edition: 1st edDescription: vii, 436 p.: ill.; 28 cmISBN: 9780879698676Subject(s): Proteins-Purification | Proteins-AnalysisDDC classification: 572.6078

Contents:

1 Introduction to protein purification strategies 2 Preparation of cellular and subcellular extracts Section 1: Purification using gel electrophoresis 3 One-dimensional polyacrylamide gel electrophoresis 4 Prparative two-dimensional gel electrophoresis with immobilized Section 2: Purification using column chromatography 5 Ion-exchange chromatography 6 Size-exclusion chromatography 7 Reversed-phase high-performance liquid chromatography 8 Affinity and immunoaffinity chromatograpphy 9 Metal chelate affinity chromatography 10 Multidimensional chromatography of intact proteins Section 3: Characterizing protein complexes 11 Identification of protein-protein interactions by conventional column chromatography 12 Identification of associated proteins by coimmunoprecipitation 13 Chromatin immunoprecipitation of protein complexe 14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins 15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis 16 In silico tools: analysis for creating focused interaction networks

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Holdings
Item type	Current library	Call number	Status	Notes	Date due	Barcode	Item holds
General Books Science Library	Science Library, Sikkim University Science Library General Section	572.6078 SIM/B (Browse shelf(Opens below))	Available	Books For SU Science Library		P03057

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1 Introduction to protein purification strategies
2 Preparation of cellular and subcellular extracts

Section 1: Purification using gel electrophoresis
3 One-dimensional polyacrylamide gel electrophoresis
4 Prparative two-dimensional gel electrophoresis with immobilized

Section 2: Purification using column chromatography
5 Ion-exchange chromatography
6 Size-exclusion chromatography
7 Reversed-phase high-performance liquid chromatography
8 Affinity and immunoaffinity chromatograpphy
9 Metal chelate affinity chromatography
10 Multidimensional chromatography of intact proteins

Section 3: Characterizing protein complexes
11 Identification of protein-protein interactions by conventional column chromatography
12 Identification of associated proteins by coimmunoprecipitation
13 Chromatin immunoprecipitation of protein complexe
14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins
15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
16 In silico tools: analysis for creating focused interaction networks

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