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The condensed protocols: from molecular cloning: a laboratory manual/ Joseph Sambrook, David W. Russell.

By: Sambrook, Joseph Contributor(s): Russell, David W Material type: Text

TextPublication details: New York: Cold Spring Harbor Laboratory Press, 2006Edition: 1st edDescription: v, 800p. 28 cmSubject(s): Molecular Cloning | Biological Techniques | Molecular BiologyDDC classification: 572.8

Contents:

1 Plasmids and their usefulness in molecular cloning 2 Bacteriophage and its vectors 3 Working with bacteriophage M13 vectors 4 Working with high-capacity vectors 5 Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis 6 Preparation and analysis of eukaryotic genomic DNA 7 Extraction, purification, and analysis of mRNA from eukaryotic cells 8 In vitro amplification of DNA by the polymerase chain reaction 9 Preparation of radiolabeled DNA by and RNA probes 10 Working with Synthetic oligonucleotide probes 11 Preparation of cDNA libraries and gene identification 12 DNA sequencing 13 Mutagenesis 14 Screening expression libraries 15 Expression of cloned genes in escherichia coli 16 Introducing cloned genes into cultured mammalian cells 17 Analysis of gene expression in mammalian cells 18 Protein interaction technologies

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Holdings
Item type	Current library	Call number	Status	Notes	Date due	Barcode	Item holds
General Books Science Library	Science Library, Sikkim University Science Library General Section	572.8 SAM/T (Browse shelf(Opens below))	Available	Books For SU Science Library		P03056

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1 Plasmids and their usefulness in molecular cloning
2 Bacteriophage and its vectors
3 Working with bacteriophage M13 vectors
4 Working with high-capacity vectors
5 Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
6 Preparation and analysis of eukaryotic genomic DNA
7 Extraction, purification, and analysis of mRNA from eukaryotic cells
8 In vitro amplification of DNA by the polymerase chain reaction
9 Preparation of radiolabeled DNA by and RNA probes
10 Working with Synthetic oligonucleotide probes
11 Preparation of cDNA libraries and gene identification
12 DNA sequencing
13 Mutagenesis
14 Screening expression libraries
15 Expression of cloned genes in escherichia coli
16 Introducing cloned genes into cultured mammalian cells
17 Analysis of gene expression in mammalian cells
18 Protein interaction technologies

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