Molecular biology techniques: a classroom laboratory manual/ (Record no. 209176)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02540cam a22001935i 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780128180242 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.8078 |
| Item number | CAR/M |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Carson, Susan. |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular biology techniques: a classroom laboratory manual/ |
| Statement of responsibility, etc. | Susan Carson, Melissa C. Srougi and D. Scott Witherow. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 4th ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | London: |
| Name of publisher, distributor, etc. | Academic Press, |
| Date of publication, distribution, etc. | 2019. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xxiv, 270 p. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Part I: Manipulation of DNA Advanced Alternatives WithinPart I 1. Getting Oriented; Practicing With Micropipettes2. Purification and Digestion of Plasmid (Vector) DNA<br/>3. Completion of Vector Preparation and Polymerase Chain Reaction Amplification of egfp <br/>4. Preparation of Insert DNA (egfp) PCR Product<br/> 5. DNA Ligation and Transformation of Escherichia coli<br/> Part II: Screening Transformants Advanced Alternatives Within <br/>Part II 6. Screening of Transformants, <br/>Part I 7. Screening of Transformants, <br/>Part II 8. Analysis of DNA Sequence From a Positive Clone,<br/> Part II Part III: Expression, Detection, and Purification of Recombinant Proteins from Bacteria Advanced Alternatives Within <br/>Part III 9. Expression of Fusion Protein from Positive Clones, SDS_PAGE and Western Blot<br/>: Part I 10. Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western Blot:<br/> Part II 11. Extraction of Recombinant Protein From Escherichia coli Using a Glutathione Affinity Column <br/>12. Analysis of Purification Fractions <br/>Part IV: Analysis of mRNA Levels<br/> 13. Total RNA Purification<br/> 14. Analysis of gst::egfp mRNA Levels by RT-qPCR:<br/> Part I 15. Analysis of gst::egfp mRNA Levels by RT-qPCR:<br/> Part II 16. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part II Part V: Modulation of Gene Expression 18. Culturing Mammalian Cells 19. Transient Transfection of Mammalian Cells 20. RNAi-Mediated Knockdown of EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II 22. RNAi-Mediated Knockdown of EGFP: Part III 23. CRISPR-Mediated Knockout of EGFP: Part I 24. CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26. Advanced CRISPR: Part II 27. Interim Laboratory Session 28. Advanced CRISPR: Part IV 29. Advanced CRISPR: Part V 30. Advanced CRISPR: Part VI Appendix A: Equipment B: Prep List C: Preparation of Competent Escherichia |
| 650 ## - SUBJECT | |
| Keyword | Molecular biology |
| General subdivision | Molecular biology--Technique |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Srougi, Melissa C. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Witherow, D. Scott. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Source of acquisition | Cost, normal purchase price | Full call number | Accession number | Date last seen | Date last checked out | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Central Library, Sikkim University | Central Library, Sikkim University | 10/08/2021 | 9 | 6683.80 | 572.8078 CAR/M | 50072 | 09/05/2024 | 25/04/2024 | General Books |