Laboratory manual of biochemistry: methods and techniques/ (Record no. 180038)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 08277nam a2200133Ia 4500 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.078 |
| Item number | SEN/L |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sengar, R.S |
| 245 #0 - TITLE STATEMENT | |
| Title | Laboratory manual of biochemistry: methods and techniques/ |
| Statement of responsibility, etc. | R.S Sengar |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New Delhi: |
| Name of publisher, distributor, etc. | NIPA, |
| Date of publication, distribution, etc. | 2014. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 344 p. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | <br/>Unit1. General Laboratory Principles 1<br/>Unit 2. Carbohydrates 11<br/>Experiment - 1 : Analysis of Carbohydrates 14<br/>Experiment - 2 : Estimation of Reducing Sugar by<br/>Dinitrosali cylate (DNS) and Plotting<br/>Standard Curve by Dextrose 23<br/>Experiment - 3 : Estimation of Sugars by Orcinol Method 25<br/>Experiment - 4 : Estimation of Reducing Sugars by<br/>Nelson-Somogyi's Method 27<br/>Experiment - 5 : Estimation Of total Soluble Sugars by<br/>Anthrone Method 30<br/>Experiment - 6 : Estimation of total Soluble Sugars By<br/>Phenol - Sulphuric Acid Method 32<br/>Experiment - 7 : Estimation of Non-Reducing Sugars 34<br/>Experiment - 8 : Estimation of Starch by Anthrone Reagent ... 36<br/>Experiment - 9 : Determination of Amylose 38<br/>Experiment - 10 : Estimation of Maltose from any Fruit<br/>Juice 40<br/>Experiment - 11 : Estimation of Cellulose from Fresh Plants .... 42<br/>Experiment - 12 : Estimation of Hemicellulose 44<br/>Experiment - 13 : Estimation of Crude Fibre 46<br/>xi<br/>Contents/xii<br/>Unit 3. Amino Acids and Proteins 49<br/>Experiment - 1 : Identification of N-terminal Amino Acid of<br/>a Protein using Dansyl Chloride Method 51<br/>Experiment - 2 : Isolation of Milk Protein (Casein) from the<br/>Milk 54<br/>Experiment - 3 : Estimation of Total Protein from Plant Fresh<br/>Tissue 56<br/>Experiment - 4 : Estimation of Total Amino Acid from Plant<br/>Fresh Tissue 58<br/>Experiment - 5 : Identify the Amino Acid present in the<br/>given sample by Qualitative Method 61<br/>Experiment - 6 : Estimate the Concentration of Amino Acids<br/>in given sample by Ninhydrin Method 65<br/>Experiment - 7 :<br/>Estimate the Concentration of Protein in<br/>given sample by Biuret Method 68<br/>Experiment - 8 :<br/>Estimate the Concentration of Protein in<br/>given sample by Folin-Low^ry's Method 71<br/>Experiment - 9 : Estimate the Concentration of Protein in<br/>given sample by Bradford's Method 74<br/>Experiment - 10 : Estimation of Amino Acid by<br/>Spectrophotometric Method 77<br/>Experiment - 11 : Native Disc-Gel Electrophoresis of<br/>Proteins 79<br/>Experiment - 12 : Separation of Proteins by SDS-PAGE 82<br/>Experiment - 13 : SDS- Polyacrylamide slab Gel<br/>Electrophoresis of Proteins under Reducing<br/>Conditions (SDS-PAGE) 86<br/>Experiment - 14 : Estimation of total Nitrogen by Micro -<br/>Kjeldahl Method 92<br/>Unit 4. Lipids 97<br/>Experiment - 1 : Extraction and Gravimetric Estimation<br/>of lipid from Ground Nut by Soxhlet<br/>Method 98<br/>Contents/xiii<br/>Experiment - 2 : Estimate the Lipid Present in given<br/>sample by Sulfo-Phospho Vanillin Method 100<br/>Experiment - 3 : Determination of Acid-Value of Fats and<br/>Oils 102<br/>Experiment - 4 : Determination of Saponification Value of<br/>Fat and Oil 105<br/>Experiment - 5 : Estimation of Free Fatty Acids 108<br/>Experiment - 6 : Determination of Iodine Value of Oil 110<br/>Experiment - 7 : Determination of Peroxide Value of Oil 112<br/>Experiment - 8 : Estimation of Volatile Acids 114<br/>Experiment - 9 : Identification and Quantification of Fatty<br/>Acids 116<br/>Experiment -10 : Steam Distillation and Isolation of Essential<br/>Oils 118<br/>Unit 5. Nucleic Acids 12i<br/>Experiment - 1 : The Determination of the Phosphorus<br/>content of a Nucleic Acid 124<br/>Experiment - 2: Estimation of DNA by Diphenylamine<br/>Experiment - 3<br/>Experiment - 4<br/>Experiment - 5<br/>(DPA) Method 125<br/>Determination of RNA by Orcinol Method ... 127<br/>To Perform Restriction Digestion of DNA .. 129<br/>To Perform Ligation of EcoRI Digest using<br/>T4 DNA Ligase 130<br/>Experiment 6:<br/>Elution of Plant DNA Fragment from<br/>Agarose Gel 132<br/>Experiment - 7: Isolation of total RNA 134<br/>Experiment - 8 : To Perform Southern Hybridization 136<br/>Experiment - 9 : To Perform<br/>PGR Amplification of Specific<br/>Target Sequence from Genomic DNA 141<br/>Experiment - 10 : To Isolate the total Genomic DNA from<br/>Plant Source 143<br/>Contents/xiv<br/>Unit 6. Enzymes<br/>Experiment - 1 : Determination of Catalytic Power of Acid<br/>Phosphatase<br/>Experiment - 2: To Obtain the Progress Curve During the<br/>Hydrolysis of p-Nitrophenyl Phosphate by<br/>Serum AlkalinePhosphatase (Ortliophospilioric<br/>Monoester Phospliohx/iirolasc, 3.1.3 152<br/>Experiment - 3: Variation in Serum Alkaline Phosphatase<br/>Activity with Enzyme Concentration 155<br/>Experiment - 4: The Determination of the Michaelis<br/>Constant for the Digestion of Casein by<br/>Trypsin (3.4.4.4.) ^60<br/>Experiment - 5 : Yeast Isocitrate Dehydrogenase: An<br/>Allosteric Enzyme ^65<br/>Experiment - 6 : Nicotinamide Adenine Dinucleotide as<br/>the Coenzyme for Lactate Dehydrogenase<br/>(L-lactate: NAD Oxidoreductase, 1.1.1.27) 168<br/>Experiment - 7: The Effect ofInhibitors on Ox Heart<br/>Lactate Dehydrogenase 173<br/>Experiment - 8 : Determination of Nitrate Reductase<br/>Activity ^78<br/>Experiment - 9 : Demonstrate the Diastase Activity in<br/>Germinating Seeds of Barley 181<br/>Experiment - 10 : The Determination of the 'Optimum<br/>Terhperature' of a-amylase 184<br/>Experiment - 11 :The<br/>Effect of Temperature on the Stability<br/>of a-amylase ^86<br/>Experiment - 12 :The pH Optimum of an Enzyme 189<br/>Experiment - 13 : Determination of Enzyme Glutamate<br/>Dehydrogenase Activity (GDH) from Plant<br/>Leaves/Shoot/Root 190<br/>Experiment - 14 : Determination of Enzyme Glutamine<br/>Synthetase Activity (GS) from Plant Tissue ..193<br/>Contents/xv<br/>Experiment - 15 : Determination of Enzyme Glutamate<br/>Synthase Activity (GOGAT) from Plant<br/>Tissue 195<br/>Experiment -16 : Estimate the Amylase Activity in the given<br/>Sample by Calculating Units<br/>(U) of Enzyme... 198<br/>Experiment - 17: investigate the Effect of Temperature<br/>on Amylase Activity and find out the<br/>Temperature Optima for Amylase 201<br/>Experiment-18 : Estimation of Catalase Enzyme from Plants....203<br/>Experiment - 19 : Estimation of Peroxidase Enzyme from<br/>Fresh Leaves 205<br/>Experiment - 20 : Estimation of Superoxide Dismutase Enzyme<br/>from Plant Tissue 207<br/>Experiment - 21: Estimation of Ascorbate Peroxidase<br/>Enzyme from Plants 211<br/>Experiment - 22: Estimation of Ascorbate Content<br/>Determination from Fresh Leaves 213<br/>Unit 7. Vitamins 217<br/>Experiment - 1 : Estimation of Ascorbic Acid (Vitamin C) by<br/>Volumetric Method 219<br/>Experiment - 2 : Estimation of Ascorbic acid (Vitamin C)<br/>by Colorimetric Method 221<br/>Estimation of Vitamin A 224<br/>Estimation of Vitamin D2 and D3 227<br/>Estimation of Tocopherol (Vitamin E) 229<br/>Estimation of Pyridoxine (B^) 232<br/>Estimation of Thiamin 235<br/>Estimation of Riboflavin 238<br/>Unit 8. Pigments 243<br/>Experiment - 1 : Estimation of<br/>Total Carotenoids from Plant<br/>Tissue 246<br/>Coiitents xvi<br/>Experiment - 2: Estimation of Chloropliyll from Plant<br/>Tissue 249<br/>Experiment - 3: To Separate Anthocynins by Paper and<br/>/or Thin layer Chromatography<br/>Experiment - 4; To Separate and Characterize the<br/>Anthocyanin Pigments<br/>Experiment - 5: Colour Development in Apples under the<br/>Influence of Quality of Light<br/>Experiment - 6: To Separate and Identify the Lycopene<br/>Present in Plant Material<br/>Experiment - 7: To Isolate the Chloroplast Pigments on the<br/>Basis of their Differential Solubility in<br/>Various Organic Solvents and theii<br/>Absorption Properties using Paper<br/>Chromatography<br/>Experiment -8: Quantitative Determinadon Study of<br/>Absorption Spectrum of Chloi ophy11s<br/>Extracted from the Leaves of Higher Plants....266<br/>Experiment - 9: Isolation of Active Chloroplasts fromExperiment observation of Hill Reaction<br/>Activity of Chloroplasts<br/>Experiment -10 :To Extract the Curcumin Pigment 270<br/>Unit 9. Phenolics<br/>Experiment -1: To Estimate Total Phenol Content in Plant ...275<br/>Experiment - 2: To Estimate the Chlorogenic Acid 277<br/>Experiment - 3: To Estimate the Tannins by Folin-Denis and<br/>Vanillin Hydroclonde Methods<br/>Experiment - 4: To Estimate the Lignins<br/>Experiment - 5; To Estimate the Capsaicin by ColonmetricExperiment gpectrophotometric Methods 285<br/><br/>Contents/xvii<br/>Unit10. Anti Nutritional Factors 289<br/>Experiment - 1 : Estimation of Trypsin Inhibitor 291<br/>Experiment - 2 : Determination of Cyanogens 293<br/>Experiment - 3 : Estimation of Phytic Acid in Food Grains .... 295<br/>Experiment - 4 : To Study the Cysteine Proteinase Inhibitor<br/>Assay 297<br/>Experiment - 5: To Study the Antifungal Activity of<br/>Cystatin Present in the Given Sample 299<br/>Unit 11. Plant Hormones<br/>Experiment - 1 : Identify the Indoleacetic Acid Present in the<br/>Given Sample 305<br/>Experiment - 2 : Estimation of Ethylene in the Plant<br/> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Date last checked out | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Central Library, Sikkim University | Central Library, Sikkim University | General Book Section | 29/08/2016 | 572.078 SEN/L | P35050 | 31/05/2024 | 31/05/2024 | General Books |