Protein Microarrays/ (Record no. 176467)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04481nam a2200145Ia 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9788182930452 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 612 |
| Item number | SIN/P |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Singh, Ram Dayal |
| 245 #0 - TITLE STATEMENT | |
| Title | Protein Microarrays/ |
| Statement of responsibility, etc. | Ram Dayal Singh |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New Delhi: |
| Name of publisher, distributor, etc. | International Scientific Publishing Academy, |
| Date of publication, distribution, etc. | 2011. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | x, 278p. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | <br/>1. PROTEIN MICROARRAYS 1<br/>Protein Therapeutics 2<br/>Applications of Protein Microarrays 3<br/>Problems and Challenges 5<br/>Sample Preparation and Handling 5<br/>Microarray Platform 7<br/>Detection Technologies 7<br/>Data Analysis 8<br/>Potential Solutions 8<br/>2. DEVELOPMENT OF A PROTEIN MICROARRAY 11<br/>Protein Microarray Technologies 13<br/>Assays on a Microspot 15<br/>Types of Spot-based Array Formats 16<br/>Capture Agents 19<br/>Solid Support and Surface Chemistry 21<br/>Arraying Devices for Immobilisation of<br/>Capture Agents 23<br/>Signal Generation and Signal Detection 24<br/>Classification Protein Microarrays 27<br/>Protein Expression Microarrays 27<br/>Protein Function Microarrays 29<br/>Protein Biomarker Screening 30<br/>Rheumatoid Arthritis 32<br/>Etiology of- Rheumatoid Arthritis 34<br/>Pathogenesis of Rheumatoid Arthritis 35<br/>Treatment of Rheumatoid Arthritis 39VIII Protein Microarra'ss<br/>3. PROTEIN MICROARRAY GENERATION<br/>AND DETECTION 42<br/>Immobilization Strategies 43<br/>Via Biotin-Avidin Interaction 44<br/>In Vitro Biotinylation of Proteins 44<br/>PCR-based In vitro Biotinylation of Proteins 47<br/>In vivo Biotinylation of Proteins 48<br/>Via N-terminal Cysteine 49<br/>Detection Strategies 50<br/>Mechanism-based Inhibitor Probes on Array 52<br/>Protein Profiling using Fluorogenic Substrates 53<br/>Array Fabrication 56<br/>4. PROTEIN STRUCTURE 58<br/>Peptide Bond 61<br/>Protein Secondary Structure 62<br/>a-Helix 62<br/>Effects of Amino Acid Sequence 65<br/>p Conformation 68<br/>Characteristic Bond Angles and Amino Acid<br/>Contents 69<br/>Protein Tertiary and Quaternary Structures 71<br/>Fibrous Proteins 72<br/>a-Keratin 73<br/>Functional Diversity in Globular Proteins 79<br/>Tertiary Structures in Globular Proteins 83<br/>Common Structural Patterns 86<br/>Structural Classification 91<br/>Protein Quaternary Structures 92<br/>5. PROPERTIES AND EVOLUTION OF PROTEINS 97<br/>Electrophoresis 100<br/>Unseparated Proteins 104<br/>Covalent Structure of Proteins 106<br/>Role ofAmino Acid Sequence 107<br/>Sequenced using Automated Procedures 109<br/>Sequence of Large Proteins 111<br/>Breaking Disulfide Bonds III<br/>Sequencing of Peptides 112<br/>Ordering Peptide Fragments US<br/>Locating Disulfide Bonds 113CONTKNTS IX<br/>Small Peptide and Proteins 114<br/>Protein Sequences and Evolution 117<br/>6. PROTEIN DENATURATION AND FOLDING 125<br/>Amino Acid Sequence Determines Tertiary Structure 127<br/>Polypeptides Fold Rapidly by a Stepwise Process 128<br/>Protein Folding 132<br/>Principles of Protein Folding 134<br/>Hydrophobic Interactions 135<br/>Experimental Evidence 136<br/>Simple Exact Models 137<br/>Salt-Induced Detour 141<br/>Triangular Scheme between D,C and N 143<br/>Using Salt to Collapse the Denatured Ensemble 145<br/>Protein Engineering Analysis of the Structure ofC 149<br/>7. FOLDING FUNNELS AND FRUSTRATION 154<br/>p-Barrel Model 1^2<br/>Signatures of Good and Bad Folders 164<br/>Thermodynamics 154<br/>Kinetics 155<br/>8. PROTEIN FOLDING PATHWAYS AND KINETICS 170<br/>Characteristics of Folding Kinetics 17g<br/>Intermediates and Folding Pathways 184<br/>Folding Speed and Topology 187<br/>Kinetic Folding Pathway of p-sheet Protein 191<br/>Stopped-flow Fluorescence Measurements 195<br/>Quenched-flow Experiments 195<br/>Equilibrium Unfolding 198<br/>Anomalous CD Overshoot Phenomenon 200<br/>Amide Proton Protection 202<br/>Hydrophobic Collapse Detected by ANS Fluorescence 204<br/>Comparison with Folding Kinetics ofInterleukin-1p 208<br/>Significance ofthe Hydrophobic Collapse 209<br/>9. PROTEIN DESIGN 212<br/>Protein Modeling 214<br/>Learning the Interaction Potentials 216<br/>Designing PDB Structures 220<br/>Similarities of Homologous Sequences 222<br/>10. evolution OFPROTEIN THERMODYNAMICS 229<br/>Evolutionary Steady-state 231X Protein Microarrans<br/>Protein Sequence-structure Compatibility 240<br/>Pfotein Structure Prediction and Sequence Design 242<br/>11. CANCER CELLS USING PROTEIN<br/>MICROARRAYS 244<br/>Cell—Lines Culture,and Irradiation 246<br/>Extraction and Delipidation 246<br/>Detection of Protein Interactions 248<br/>Analysis of Differential Protein Expression 248<br/>Imunoblot Analysis 250<br/>Qualitative Protein Levels in Antibody Microarrays 250<br/>12. VALIDATION OF MICROARRAYS 260<br/>AimsofValidation 260<br/>Challenges ofValidation 261<br/>Validation in Silico 264<br/>Validation using PCR 268<br/>Qualitative PCR 269<br/>Semi-Quantitative PCR 270<br/>Real-time Quantitative PCR 273<br/>Double-stranded DNA-binding<br/>Agents 273<br/>Validation Using Protein Antibodies 277 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Date last checked out | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Central Library, Sikkim University | Central Library, Sikkim University | General Book Section | 29/08/2016 | 612 SIN/P | P31467 | 29/05/2023 | 26/05/2023 | General Books |