Plant tissue culture: techniques and experiments/ (Record no. 176429)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 00388nam a2200145Ia 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9789382291763 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 630 |
| Item number | SMI/P |
| 245 #0 - TITLE STATEMENT | |
| Title | Plant tissue culture: techniques and experiments/ |
| Statement of responsibility, etc. | Smith, Roberta H. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 3rd ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Amsterdam: |
| Name of publisher, distributor, etc. | Elsevier, |
| Date of publication, distribution, etc. | 2013. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xvi,188p. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | <br/>Scheduling and Interrelationships of Exercises<br/>History of Plant Cell Culture<br/>Trevor A. Thorpe<br/>Introduction<br/>The Early Years<br/>The Era of Techniques Development<br/>The Recent Past<br/>Cell Behavior<br/>Plant Modification and Improvement<br/>Pathogen-Free Plants and Germplasm Storage<br/>Clonal Propagation<br/>Product Formation<br/>The Present Era<br/>Setup of a Tissue Culture Laboratory<br/>Work Areas<br/>Media Preparation/Culture Evaluation/Record-Keeping Area<br/>Aseptic Transfer Area<br/>Environmentally Controlled Culture Area<br/>Media Components and Preparation<br/>Inorganic Salts<br/>Plant Growth Regulators<br/>Vitamins<br/>Carbohydrates<br/>Hexitols<br/>Gelling Agent<br/>Amino Acids<br/>Antibiotics<br/>Natural Complexes<br/>Media pH<br/>Medium Preparation<br/>VI Contents<br/>4. Explant Preparation 45<br/>Explant Age 46<br/>Season 46<br/>Explant Size 46<br/>Plant Quality 46<br/>Goal 46<br/>Genotype 48<br/>Aseptic Technique 48<br/>Aseptic Germination of Seeds 49<br/>Carrot, Sunflower, Broccoli, Cotton, or Any Seed of Choice 49<br/>Douglas Fir 50<br/>5. Contamination 53<br/>Explant 53<br/>Disinfecting Agents 54<br/>Explant Source 56<br/>Internal Microbial Contamination 56<br/>Explant Pretreatment to Control Microbial Contaminants 57<br/>Personnel 57<br/>Laminar Air Flow Hood 58<br/>Insects 58<br/>Control of Insect Contamination 58<br/>Media 59<br/>Instruments 60<br/>Room Air Handling System 61<br/>6. Callus Induction 63<br/>Callus Initiation 63<br/>Explant Preparation 65<br/>Culture 66<br/>Observations 66<br/>Data and Questions 66<br/>Explant Orientation 67<br/>Explant 67<br/>Observations 68<br/>Establishment of Competent Cereal Cell Cultures 69<br/>Explants 70<br/>Data and Questions 70<br/>Salt Selection In Vitro 70<br/>Explants 71<br/>Data and Questions 72<br/>Growth Curves 72<br/>Cotton 73<br/>Tobacco 74<br/>Cellular Variation from Callus Cultures 76<br/>Contents vil<br/>8.<br/>9.<br/>Explant<br/>76<br/>Data and Questions<br/>76<br/>Regeneration and Morphogenesis<br/>81<br/>Controlled Morphogenesis: Potato Tuberization 82<br/>Explant 83<br/>Data and Questions 84<br/>Somatic Embryogenesis<br/>84<br/>Explant Preparation<br/>86<br/>Procedure 86<br/>Data and'Questions<br/>86<br/>Regeneration of Rice<br/>87<br/>Regeneration<br/>87<br/>Data and Questions<br/>87<br/>Dormancy Requirements of Explants<br/>87<br/>Explant Preparation<br/>88<br/>Questions<br/>89<br/>Primary Morphogenesis: Douglas Fir<br/>89<br/>Explant Preparation<br/>89<br/>Data and Questions<br/>90<br/>Woody Shrubs and Trees 93<br/>Brent H. McCown<br/>Rhododendrons and Azaleas<br/>^5<br/>Explants<br/>97<br/>Advanced Studies<br/>97<br/>Roses<br/>97<br/>Explant<br/>98<br/>Advanced Studies<br/>98<br/>Birch Trees<br/>98<br/>Explant<br/>98<br/>Advanced Studies<br/>99<br/>White Cedar (Arborvitae)<br/>99<br/>Explant<br/>99<br/>Haploid Plants from Anther Culture<br/>103<br/>Datura Anther Culture<br/>105<br/>Explant Preparation<br/>105<br/>Questions<br/>106<br/>African Violet<br/>106<br/>Explant Preparation<br/>107<br/>Observations<br/>107<br/>Root Tip Chromosome Squash Technique<br/>107<br/>Questions<br/>108<br/>Tobacco<br/>108<br/>VIII<br/>10.<br/>Explant Preparation<br/>108<br/>Observations<br/>109<br/>Questions<br/>109<br/>Anther Squash Technique<br/>109<br/>Embryo Rescue<br/>113<br/>Sweet Corn Embryo Culture<br/>114<br/>Preparation and Culture of Corn Embryos<br/>115<br/>Questions<br/>Crabapple and Pear<br/>116<br/>Explant Preparation<br/>Observations<br/>Questions<br/>Meristem Culture for Virus-Free Plants<br/>119<br/>Isolation of the Shoot Apical Meristem<br/>Explant Preparation<br/>Questions<br/>Establishing Virus- and Bacteria-Free Plants<br/>122<br/>Explant Preparation<br/>122<br/>Garlic Propagation from Bulb Scales<br/>123<br/>Explant Preparation<br/>123<br/>Data<br/>123<br/>In Vitro Propagation for Commercial Production of<br/>Ornamentals<br/>127<br/>Boston Fern<br/>Stage 1<br/>Stage 1!<br/>Stage 111<br/>Staghorn Fern<br/>133<br/>Stage 1<br/>Stage II<br/>134<br/>Stage HI<br/>135<br/>Ficus<br/>136<br/>Stage !<br/>136<br/>Stage II<br/>Stage III<br/>137<br/>Kalanchoe<br/>Explant Preparation<br/>138<br/>Data and Questions<br/>138<br/>African Violet<br/>139<br/>Explant Preparation<br/>139<br/>Data and Questions<br/>140<br/>Endangered Species<br/>Pitcher Plants 141<br/>Cactus -|4-|<br/>Explant Preparation 142<br/>13. Protoplast Isolation and Fusion 147<br/>Jungeun Kim Park, Sunghun Park and James E. Craven<br/>Plant Protoplast Isolation and Fusion 148<br/>Supplies 148<br/>Protoplast Liberation 149<br/>Protoplast Purification 150<br/>Vital Staining 151<br/>Protoplast Fusion 151<br/>Yield Determination 15I<br/>Developing a Protoplast Isolation Protocol 152<br/>14. /\grobacfer/t/m-Mediated Transformation of Plants 155<br/>Jungeun Kim Park, Sunghun Park, Qingyu Wu and Stuart Sprague<br/>Petunia or Tobacco Leaf Disk ^ I57<br/>Sequence I57<br/>Preculture 157<br/>Explant Preparation I57<br/>Cocultivation with Agrobacterium 158<br/>Shoot Transformation and Elimination Agrobacterium 158<br/>Rooting and Pretesting For Transformed Shoots 159<br/>Petunia Shoot Apex 160<br/>Sequence 160<br/>Seed Germination 160<br/>Shoot Induction 161<br/>Shoot Apex Transformation 161<br/>Rooting 161<br/>Tobacco Leaf Infiltration 162<br/>Sequence * 162<br/>Crow Agrobacterium 162<br/>Culture Agrobacterium 163<br/>Harvest Agrobacterium 163<br/>Infiltration Tobacco Leaves 163<br/> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Central Library, Sikkim University | Central Library, Sikkim University | General Book Section | 29/08/2016 | 630 SMI/P | P31429 | 29/08/2016 | General Books |