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Methods in yeast genetics: a cold spring harbor laboratory course manual/ (Record no. 170474)

MARC details
000 -LEADER
fixed length control field	00429nam a2200145Ia 4500
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number	97808796697280
040 ## - CATALOGING SOURCE
Transcribing agency	CUS
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	579.562
Item number	AMB/M
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name	Amberg, David C.
245 #0 - TITLE STATEMENT
Title	Methods in yeast genetics: a cold spring harbor laboratory course manual/
Statement of responsibility, etc.	David C. Amberg, Daniel J. Burke, Jeffrey N. Strathern.
250 ## - EDITION STATEMENT
Edition statement	1st ed.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc.	New York:
Name of publisher, distributor, etc.	Cold spring harbor lab.press,
Date of publication, distribution, etc.	2005.
300 ## - PHYSICAL DESCRIPTION
Extent	xvii, 230 p. :
Other physical details	ill. ;
Dimensions	28 cm.
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note	Experiments<br/>1 Looking at Yeast Cells<br/>2 Isolation and Charaterization of Auxotrophic, Temperature-sensitive, and Osmotic-sensitive Mutants<br/>3 Meitotic Mapping<br/>4 Mitotic Recombination and Random Spore Analysis<br/>5 Transformation of Yeast<br/>6 Synthetic Lethal Mutants<br/>7 Gene Replacement<br/>8 Isolation of ras2 Suppressors<br/>9 Manipulating Cell Types<br/>10 Isolating Mutants by Insertional Mutagenesis<br/>11 Isolation of Separation of Function Mutants by Two-hybrid Differential Interaction Screening<br/><br/>Techniques and Protocols<br/>1 High-efficiency Transformation of Yeast<br/>2 " Quick and Dirty" Plasmid Transformation of Yeast Colonies <br/>3 Yeast DNA Isolation<br/>A Yeast DNA Miniprep (40 ml)<br/>B Yeast DNA Miniprep (5 ml)<br/>C A Ten-minute DNA Preparation from Yeast<br/>D Yeast Genomic DNA: Glass-bead Preparation<br/>4 Yeast Protein Extracts<br/>5 TAP Purification<br/>6 Yeast RNA Isolations<br/>7 Hydroxylamine Mutagenesis of Plansmid DNA<br/>8 Assay of B-Galactosidase in Yeast<br/>9 Plate Assay for Carboxypeptidase Y<br/>10 Random Spore Analysis<br/>11 Yeast Vital Stains<br/>A DAPI Stain of Nuclear and Mitochondrial DNA<br/>B Visualizing Mitochondria with DiOC6 or DilC5(3)<br/>C Visualizing vacuoles and Endocytic Compartments with FM4-64<br/>D Calcofluor Staining of Chitin and Bud Scars<br/>12 Yeast Immunofluorescence<br/>13 Actin Staining in Fixed Cells<br/>14 PCR-mediated Gene Disruption<br/>A One-step PCR Gene Disruption<br/>B Gene Disruption by Double-fusion PCR<br/>15 Yeast Colony PCR<br/>16 Measuring Yeast Cell Density by Spectrophotometry<br/>17 Cell Synchrony<br/>18 Chromatin Immunoprecipitation<br/>19 Flow Cytometry of Yeast DNA<br/>20 Logarithmic Growth<br/>21 EMS Mutagensis<br/>22 Tetrad Dissection<br/>23 Making a Tetrad Dissection Needle<br/>24 Picking Zygotes<br/>25 Determining Plating Efficiency<br/>26 DNA Miniprep from E. Coli<br/>27 Preparing and Transfroming Competent E. Coli<br/>28 Storing and Handling the Systematic Deletion Collection
650 ## - SUBJECT
Keyword	Yeasts
General subdivision	Genetics
Geographic subdivision	Laboratory Manuals
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Burke, Daniel J.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Strathern, Jeffrey N.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	General Books Science Library

Holdings
Withdrawn status	Lost status	Damaged status	Not for loan	Home library	Current library	Shelving location	Date acquired	Full call number	Accession number	Date last seen	Koha item type	Public note
				Science Library, Sikkim University	Science Library, Sikkim University	Science Library General Section	27/10/2016	579.562 AMB/M	P25452	16/01/2020	General Books Science Library	Books For SU Science Library