Molecular biology and biotechnology: Microbial methods/ (Record no. 166834)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 00358nam a2200133Ia 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9380235194 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.8 |
| Item number | PAR/M |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Parakhia, Manoj V. [et al.]. |
| 245 #0 - TITLE STATEMENT | |
| Title | Molecular biology and biotechnology: Microbial methods/ |
| Statement of responsibility, etc. | Manoj V. Parakhia [et al.]. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New Delhi: |
| Name of publisher, distributor, etc. | NIPA, |
| Date of publication, distribution, etc. | 2010. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xix, 360p.: |
| Other physical details | ill.; |
| Dimensions | 25 cm. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 1. Laboratory safety rules<br/>2. Milestones in DNA history and biotechnology<br/>3. Contribution in development of DNA and biotechnology<br/>4. Equipments required in a molecular marker laboratory<br/>5. Basic calculations used in microbial biotechnology<br/>6. Basic principals in preparation of reagents required in microbial biotechnology<br/>7. Isolation of bacterial genomic DNA<br/>8. Isolation of RNA from yeast<br/>9. Nucleotide composition of RNA<br/>10. DNA melting point determination<br/>11. Quantitative determination of DNA by DPA method and spectroscopic method<br/>12. Estimation of RNA by Orcinol method<br/>13. Electrophoresis<br/>14. SDS-PAGE electrophoresis<br/>15. Polymerase chain reaction<br/>16. Restriction enzyme digestion<br/>17. Extraction of DNA fragments from Agarose Gel<br/>18. Isolation the Mammalian DNA from whole blood<br/>19. Isolation of Plasmid DNA from bacteria<br/>20. Random Amplified Polymorphic DNA RAPD<br/>21. Amplified Fragment Leanth Polymorphism AFLP<br/>22. Southern blotting<br/>23. Ligation of insert DNA to Vector DNA<br/>24. Bacterial transformation<br/>25. Detection of plasmid by transformation<br/>26. Conjugation in E.Coli by plate method<br/>27. Isolation of Lactose non fermentor mutants of E.Coli by Physical mutagenesis<br/>28. Isolation of auxotrophic mutant by chemical 5 Bu mutagenesis<br/>29. Mutagenesis direct selection<br/>30. Mutagenesis indirect selection<br/>31. Agrobacterium mediated gene transfer from bacteria to plant. 32. Western blotting<br/>33. Northern blotting<br/>34. PCR-sequencing<br/>35. Overview of metagenomics<br/>36. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0. |
| 650 ## - SUBJECT | |
| Keyword | Molecular Biology |
| 650 ## - SUBJECT | |
| Keyword | Biotechnology |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books Science Library |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Koha item type | Public note |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Science Library, Sikkim University | Science Library, Sikkim University | Science Library General Section | 29/08/2016 | 572.8 PAR/M | P21767 | 16/01/2020 | General Books Science Library | Books For SU Science Library |