Basic methods in protein purification and analysis: a laboratory manual/ (Record no. 148399)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 00360nam a2200121Ia 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780879698676 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.6078 |
| Item number | SIM/B |
| 245 #0 - TITLE STATEMENT | |
| Title | Basic methods in protein purification and analysis: a laboratory manual/ |
| Statement of responsibility, etc. | edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New York: |
| Name of publisher, distributor, etc. | Cold Spring Harbor Laboratory Press, |
| Date of publication, distribution, etc. | 2009. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | vii, 436 p.: |
| Other physical details | ill.; |
| Dimensions | 28 cm. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 1 Introduction to protein purification strategies<br/>2 Preparation of cellular and subcellular extracts<br/><br/>Section 1: Purification using gel electrophoresis<br/>3 One-dimensional polyacrylamide gel electrophoresis<br/>4 Prparative two-dimensional gel electrophoresis with immobilized<br/><br/>Section 2: Purification using column chromatography<br/>5 Ion-exchange chromatography<br/>6 Size-exclusion chromatography<br/>7 Reversed-phase high-performance liquid chromatography<br/>8 Affinity and immunoaffinity chromatograpphy<br/>9 Metal chelate affinity chromatography<br/>10 Multidimensional chromatography of intact proteins<br/><br/>Section 3: Characterizing protein complexes<br/>11 Identification of protein-protein interactions by conventional column chromatography <br/>12 Identification of associated proteins by coimmunoprecipitation<br/>13 Chromatin immunoprecipitation of protein complexe<br/>14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins<br/>15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis<br/>16 In silico tools: analysis for creating focused interaction networks<br/> |
| 650 ## - SUBJECT | |
| Keyword | Proteins-Purification |
| 650 ## - SUBJECT | |
| Keyword | Proteins-Analysis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | edited by Simpson, Richard J. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Adams, Peter D. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Golemis, Erica A. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books Science Library |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Date last checked out | Koha item type | Public note |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Science Library, Sikkim University | Science Library, Sikkim University | Science Library General Section | 28/08/2016 | 572.6078 SIM/B | P03057 | 16/01/2020 | 20/11/2019 | General Books Science Library | Books For SU Science Library |