Molecular biology techniques: a classroom laboratory manual/
Carson, Susan.
Molecular biology techniques: a classroom laboratory manual/ Susan Carson, Melissa C. Srougi and D. Scott Witherow. - 4th ed. - London: Academic Press, 2019. - xxiv, 270 p.
Part I: Manipulation of DNA Advanced Alternatives WithinPart I 1. Getting Oriented; Practicing With Micropipettes2. Purification and Digestion of Plasmid (Vector) DNA
3. Completion of Vector Preparation and Polymerase Chain Reaction Amplification of egfp
4. Preparation of Insert DNA (egfp) PCR Product
5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening Transformants Advanced Alternatives Within
Part II 6. Screening of Transformants,
Part I 7. Screening of Transformants,
Part II 8. Analysis of DNA Sequence From a Positive Clone,
Part II Part III: Expression, Detection, and Purification of Recombinant Proteins from Bacteria Advanced Alternatives Within
Part III 9. Expression of Fusion Protein from Positive Clones, SDS_PAGE and Western Blot
: Part I 10. Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western Blot:
Part II 11. Extraction of Recombinant Protein From Escherichia coli Using a Glutathione Affinity Column
12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels
13. Total RNA Purification
14. Analysis of gst::egfp mRNA Levels by RT-qPCR:
Part I 15. Analysis of gst::egfp mRNA Levels by RT-qPCR:
Part II 16. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part II Part V: Modulation of Gene Expression 18. Culturing Mammalian Cells 19. Transient Transfection of Mammalian Cells 20. RNAi-Mediated Knockdown of EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II 22. RNAi-Mediated Knockdown of EGFP: Part III 23. CRISPR-Mediated Knockout of EGFP: Part I 24. CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26. Advanced CRISPR: Part II 27. Interim Laboratory Session 28. Advanced CRISPR: Part IV 29. Advanced CRISPR: Part V 30. Advanced CRISPR: Part VI Appendix A: Equipment B: Prep List C: Preparation of Competent Escherichia
9780128180242
Molecular biology--Molecular biology--Technique
572.8078 / CAR/M
Molecular biology techniques: a classroom laboratory manual/ Susan Carson, Melissa C. Srougi and D. Scott Witherow. - 4th ed. - London: Academic Press, 2019. - xxiv, 270 p.
Part I: Manipulation of DNA Advanced Alternatives WithinPart I 1. Getting Oriented; Practicing With Micropipettes2. Purification and Digestion of Plasmid (Vector) DNA
3. Completion of Vector Preparation and Polymerase Chain Reaction Amplification of egfp
4. Preparation of Insert DNA (egfp) PCR Product
5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening Transformants Advanced Alternatives Within
Part II 6. Screening of Transformants,
Part I 7. Screening of Transformants,
Part II 8. Analysis of DNA Sequence From a Positive Clone,
Part II Part III: Expression, Detection, and Purification of Recombinant Proteins from Bacteria Advanced Alternatives Within
Part III 9. Expression of Fusion Protein from Positive Clones, SDS_PAGE and Western Blot
: Part I 10. Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western Blot:
Part II 11. Extraction of Recombinant Protein From Escherichia coli Using a Glutathione Affinity Column
12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels
13. Total RNA Purification
14. Analysis of gst::egfp mRNA Levels by RT-qPCR:
Part I 15. Analysis of gst::egfp mRNA Levels by RT-qPCR:
Part II 16. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part II Part V: Modulation of Gene Expression 18. Culturing Mammalian Cells 19. Transient Transfection of Mammalian Cells 20. RNAi-Mediated Knockdown of EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II 22. RNAi-Mediated Knockdown of EGFP: Part III 23. CRISPR-Mediated Knockout of EGFP: Part I 24. CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26. Advanced CRISPR: Part II 27. Interim Laboratory Session 28. Advanced CRISPR: Part IV 29. Advanced CRISPR: Part V 30. Advanced CRISPR: Part VI Appendix A: Equipment B: Prep List C: Preparation of Competent Escherichia
9780128180242
Molecular biology--Molecular biology--Technique
572.8078 / CAR/M