DNA Protein Interactions: principles and protocols/
DNA Protein Interactions: principles and protocols/
edited by Tom Moss and Benoit Leblanc.
- 3rd ed.
- New York: Humana Press, 2009.
- xvii, 680p.: ill.; 28 cm.
1. Filter-Binding Assays
2. Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
3. DNase I Footprinting
4. Exonuclease III Footprinting on Immobilized DNA Templates 5. Hydroxyl Radical Footprinting of Protein-DNA Complexes
6. The Use of Diethyl Pyrocarbonate (DEPC) and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA
7. Uranyl Photofootprinting
8. Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
9. Ethylation Interference Footprinting of DNA-Protein Complexes
10. Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates within Model Chromatin Complexes
11. Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX
12. Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
13. Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper
14. Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots
15. Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions
16. Chromatin Immunoprecipitation in Mammalian Cells
17. Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP
18. Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae
19. Nucleosome Mapping
20. In Cellulo DNA Analysis (LMPCR Footprinting)
21. Atomic Force Microscopy Imaging and Probing of DNA, Proteins and Protein-DNA Complexes: Silatrane Surface Chemistry
22. Two-Dimensional Crystallisation of Soluble Protein Complexes
23. Assays for Transcription Factor Activity
24. Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
25. Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes
26. Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex
27. Site-Directed DNA Cross-Linking of Large Multi-Subunit Protein-DNA Complexes
28. Functional Studies of DNA-Protein Interactions Using FRET Techniques
29. Single-Molecule FRET Analysis of Protein-DNA
30. Analysis of DNA Supercoiling Induced by DNA-Protein Interactions
31. The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA
32. Plasmid Vectors for the Analysis of Protein Induced DNA-Bending
33. Analysis of Distant Communication on Defined Chromatin Templates In Vitro
34. A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
35. Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions
36. Circular Dichroism for the Analysis of Protein-DNA Interactions
37. Defining the Thermodynamics of Protein/DNA Complexes and Their Components Using Micro-Calorimetry
38. Surface Plasmon Resonance Assays of DNA-Protein Interactions
9781603270144
DNA-Protein Interactions
Human Genetics
572.86 / MOS/D
1. Filter-Binding Assays
2. Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
3. DNase I Footprinting
4. Exonuclease III Footprinting on Immobilized DNA Templates 5. Hydroxyl Radical Footprinting of Protein-DNA Complexes
6. The Use of Diethyl Pyrocarbonate (DEPC) and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA
7. Uranyl Photofootprinting
8. Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
9. Ethylation Interference Footprinting of DNA-Protein Complexes
10. Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates within Model Chromatin Complexes
11. Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX
12. Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
13. Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper
14. Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots
15. Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions
16. Chromatin Immunoprecipitation in Mammalian Cells
17. Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP
18. Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae
19. Nucleosome Mapping
20. In Cellulo DNA Analysis (LMPCR Footprinting)
21. Atomic Force Microscopy Imaging and Probing of DNA, Proteins and Protein-DNA Complexes: Silatrane Surface Chemistry
22. Two-Dimensional Crystallisation of Soluble Protein Complexes
23. Assays for Transcription Factor Activity
24. Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
25. Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes
26. Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex
27. Site-Directed DNA Cross-Linking of Large Multi-Subunit Protein-DNA Complexes
28. Functional Studies of DNA-Protein Interactions Using FRET Techniques
29. Single-Molecule FRET Analysis of Protein-DNA
30. Analysis of DNA Supercoiling Induced by DNA-Protein Interactions
31. The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA
32. Plasmid Vectors for the Analysis of Protein Induced DNA-Bending
33. Analysis of Distant Communication on Defined Chromatin Templates In Vitro
34. A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
35. Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions
36. Circular Dichroism for the Analysis of Protein-DNA Interactions
37. Defining the Thermodynamics of Protein/DNA Complexes and Their Components Using Micro-Calorimetry
38. Surface Plasmon Resonance Assays of DNA-Protein Interactions
9781603270144
DNA-Protein Interactions
Human Genetics
572.86 / MOS/D