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Basic methods in protein purification and analysis: a laboratory manual/ edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis - 1st ed. - New York: Cold Spring Harbor Laboratory Press, 2009. - vii, 436 p.: ill.; 28 cm.

1 Introduction to protein purification strategies
2 Preparation of cellular and subcellular extracts

Section 1: Purification using gel electrophoresis
3 One-dimensional polyacrylamide gel electrophoresis
4 Prparative two-dimensional gel electrophoresis with immobilized

Section 2: Purification using column chromatography
5 Ion-exchange chromatography
6 Size-exclusion chromatography
7 Reversed-phase high-performance liquid chromatography
8 Affinity and immunoaffinity chromatograpphy
9 Metal chelate affinity chromatography
10 Multidimensional chromatography of intact proteins

Section 3: Characterizing protein complexes
11 Identification of protein-protein interactions by conventional column chromatography
12 Identification of associated proteins by coimmunoprecipitation
13 Chromatin immunoprecipitation of protein complexe
14 Identification of protein-protein interactions with glutathione-S-transferase fusion proteins
15 Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
16 In silico tools: analysis for creating focused interaction networks


ISBN: 9780879698676

Subjects--Topical Terms:
Proteins-Purification
Proteins-Analysis

Dewey Class. No.: 572.6078 / SIM/B